對硝基苯磷酸二鈉鹽 產(chǎn)品描述
pNPP-Na鹽(p-nitrophenyl phosphate, disodium salt,對-硝基苯磷酸二鈉)在ELISA應(yīng)用中作為檢測堿性磷酸酶的底物而被廣泛使用。當(dāng)堿性磷酸酶與pNPP反應(yīng)后,形成黃色水溶性反應(yīng)產(chǎn)物,加入 3 N NaOH可終止反應(yīng),該產(chǎn)物在405nm有光吸收。
對硝基苯磷酸二鈉鹽 注意事項(xiàng)
為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
溶解方法
<!--[if !supportLists]-->1. <!--[endif]-->pNPP使用前先平衡至室溫。
<!--[if !supportLists]-->2. <!--[endif]-->用5ml 10mM二乙醇胺緩沖液(含0.5mM MgCl2,pH9.5)溶解一片pNPP-Na鹽(即5mg),終濃度為1mg/ml。混勻,并避光。
<!--[if !supportLists]-->3. 為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
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