BAFF-R Elisa試劑盒是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法,由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢。
靈敏度,檢測(cè)范圍,特異性和重復(fù)性:
靈敏度:zui小可測(cè) 0.094ng/mL。
檢測(cè)范圍:0.156–10ng/mL。
特異性:可檢測(cè)重組或天然的待測(cè)物質(zhì),且與其它相關(guān)蛋白無交叉反應(yīng)。
重復(fù)性:板內(nèi),板間變異系數(shù)均<10%。
BAFF-R ELISA分析檢測(cè)試劑盒,英文名稱:BAFF-R ELISA Kit,常用網(wǎng)絡(luò)搜索名:BAFF-R ELISA試劑盒,規(guī)格:48T/96T。
BAFF-R Elisa試劑盒間接法測(cè)抗體流程示意圖:
BAFF-R Elisa試劑盒樣本稀釋原則:
首先通過文獻(xiàn)檢索的方式了解待測(cè)樣本的大致含量,確定適當(dāng)?shù)南♂尡稊?shù)。只有稀釋至標(biāo)準(zhǔn)曲線的范圍內(nèi),檢測(cè)的結(jié)果才是準(zhǔn)確的。稀釋的過程中,應(yīng)做好詳細(xì)的記錄。zui后計(jì)算濃度時(shí),稀釋了“N”倍,標(biāo)本的濃度應(yīng)再乘以“N”。
(1)、標(biāo)準(zhǔn)品的稀釋原則:2瓶,每瓶臨用前以樣品稀釋液稀釋至1ml,蓋好后靜置10分鐘以上,然后反復(fù)顛倒/搓動(dòng)以助溶解,其濃度為300 pg/ml,做系列倍比稀釋后,分別稀釋300 pg/ml,150 pg/ml,75 pg/ml,37.5 pg/ml,18.5 pg/ml,9 pg/ml,4.5 pg/ml,樣品稀釋液直接作為標(biāo)準(zhǔn)濃度0 pg/ml,臨用前15分鐘內(nèi)配制。
如配制150 pg/ml標(biāo)準(zhǔn)品:取0.5ml(不要少于0.5ml)300 pg/ml的上述標(biāo)準(zhǔn)品加入含0.5ml樣品稀釋液的Eppendorf管中,混勻即可,其余濃度以此類推。
(2)、BAFF-R Elisa試劑盒*標(biāo)記抗體的稀釋原則:臨用前以*標(biāo)記抗體稀釋液稀釋,稀釋前根據(jù)預(yù)先計(jì)算好的每次實(shí)驗(yàn)所需的總量配制(每孔100μl),實(shí)際配制時(shí)應(yīng)多配制0.1-0.2ml。如10μl*標(biāo)記抗體加990μl*標(biāo)記抗體稀釋液的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制。
(3)、辣根過氧化物酶標(biāo)記親和素的稀釋原則:臨用前以辣根過氧化物酶標(biāo)記親和素稀釋液稀釋,稀釋前根據(jù)預(yù)先計(jì)算好的每次實(shí)驗(yàn)所需的總量配制(每孔100μl),實(shí)際配制時(shí)應(yīng)多配制0.1-0.2ml。如10μl辣根過氧化物酶標(biāo)記親和素加990μl辣根過氧化物酶標(biāo)記親和素稀釋液 的比例配制,輕輕混勻,在使用前一小時(shí)內(nèi)配制。
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