Anti-RBP4抗體,*結(jié)合蛋白4抗體一抗孵育(Primary antibody incubation):
參考一抗的說明書,按照適當(dāng)比例用Western一抗稀釋液稀釋一抗。
用微型臺式真空泵吸盡封閉液,立即加入稀釋好的一抗,室溫或4℃在側(cè)擺搖床上緩慢搖動孵育一小時。如果一抗孵育一小時效果不佳,可以在4℃緩慢搖動孵育過夜?;厥找豢?。加入Western洗滌液,在側(cè)擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長洗滌時間并增加洗滌次數(shù)。
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產(chǎn)品名稱:Anti-RBP4抗體,*結(jié)合蛋白4抗體
產(chǎn)品編號:BYK-6182R
英文名稱:Anti-RBP4
中文名稱:*結(jié)合蛋白4抗體
英文別名:plasma retinol binding protein 4; Plasma retinol-binding proteinv; Plasma retinol-binding protein(1-176); prbp; PRO2222; RBP; RBP4; RET4_HUMAN; Retinol binding protein 4; retinol binding protein 4 interstitial; Retinol binding protein 4 plasma.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-RBP4抗體,*結(jié)合蛋白4抗體
英文介紹:This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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