小鼠脂蛋白α(Lp-α)ELISA試劑盒
英文名稱:Mouse lipoprotein α,Lp-α ELISA Kit
貨號:ZK-M5104
規(guī)格: 96T/48T
特點(diǎn):靈敏性高、特異性強(qiáng)、重復(fù)性好
服務(wù):子科生物所有的elisa試劑盒均可免費(fèi)代測,全程Elisa實(shí)驗(yàn)技術(shù)指導(dǎo)。
小鼠脂蛋白α(Lp-α)ELISA試劑盒實(shí)驗(yàn)原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、生物素化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過*洗滌后用底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的該指標(biāo)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),計(jì)算樣品濃度。
小鼠脂蛋白α(Lp-α)ELISA試劑盒
標(biāo)本的采集及保存
1. 血清:全血標(biāo)本請于室溫放置2小時(shí)或4℃過了夜后于1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
3.細(xì)胞培養(yǎng)物上清或其它生物標(biāo)本:1000 x g離心20分鐘,取上清即可檢測,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
注:標(biāo)本溶血會(huì)影響zui后檢測結(jié)果,因此溶血標(biāo)本不宜進(jìn)行此項(xiàng)檢測。
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