人腸堿性磷酸酶(ALPI)ELISA檢測試劑盒子科生物現(xiàn)貨供應(yīng),子科生物專業(yè)生產(chǎn)研發(fā)經(jīng)營穩(wěn)定、優(yōu)質(zhì)、高效、實(shí)用的ELISA試劑盒,本公司可以提供ELISA試劑盒免費(fèi)代測,ELISA試劑盒批發(fā)價零售,是ELISA試劑盒專業(yè)生產(chǎn)廠家,并代理各大進(jìn)口原裝品牌ELISA試劑盒,貨期短,*,原裝正品,!
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人腸堿性磷酸酶(ALPI)ELISA檢測試劑盒操作步驟:
1. 使用前,將所有試劑充分混勻。不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差。
2. 根據(jù)待測樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù)。每個標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔。每個樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔。
3. 加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測樣品50ul于反應(yīng)孔內(nèi)。立即加入50ul的生物素標(biāo)記的抗體。蓋上膜板,輕輕振蕩混勻,37℃溫育45分鐘。
4. 甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干。重復(fù)此操作4次。如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次。
5. 每孔加入100ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘。
6. 甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干。重復(fù)此操作4次。如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次。
7. 每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育5分鐘。避免光照。
8. 取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測定結(jié)果。
9. 在450nm波長處測定各孔的OD值。
結(jié)果判斷與分析:
1、 儀器值:于波長450nm的酶標(biāo)儀上讀取各孔的OD值
2、 以吸光度OD值為縱坐標(biāo)(Y),相應(yīng)的LTB4標(biāo)準(zhǔn)品濃度為橫坐標(biāo)(X),做得相應(yīng)的曲線,樣品的LTB4含量可根據(jù)其OD值由標(biāo)準(zhǔn)曲線換算出相應(yīng)的濃度,再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)準(zhǔn)曲線的回歸方程式,將樣品的OD值代入方程式,計算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實(shí)際濃度。
人腸堿性磷酸酶(ALPI)ELISA檢測試劑盒9017112 Kit, Service Prot. Plasticware, BR8 RD
9017091 Uprade kit, RoboVac base BR8 RD v4.2
9232335 Cable, pump, DISU, BR8000
9238771 Mission Pack Includes 6 working days (on-site or off-site work) by a QIAGEN Instrumentation Service Specialist, valid for 1 year. These days can be used for writing and/or optimization of protocols or for training. It includes all travel and labor charges. Parts, consumable chemistries, and accessories are not included. Custom protocols are the sole responsibility of the customer and neither warrantied nor guaranteed
9238919 Tooth belt 330mm, BS15
9238910 Disc tray, BS96
9238700 Front panel w. keyboard foil (Zent2)
9238693 Voltage regulator 7805 DC, 15 V
9232099 Front blind for size 120
9230968 Holder, X-drive motor, BR8000
9014724 DISU Retraction Mechanism BRMDx,complete
9014723 Scanner, Complete, SamTrack, BR8 RD
9014580 Vacuum Manifold, SBS
9014525 Modules, DISU, BR8 RD v1.0
9022576 Strut assembly, Hood, AS, right, Qsym
9022338 DVD, System Restore, DCU
9021982 Gas strut, door, QXA
9021614 Kit, upgrade QSAS Cabinet, Qsym
9021282 Drop catcher, waste chute, v1, Qsym
9238177 QIAplate Holder, silver
9240647 Internal BCR, MS-3 CCD, AP196/98
9240637 UPS, 240 Volt, AP
9240629 Supplemental Fuse Pack, AP196/98
9240618 Fuse, TE5, 1.6A, PCB, AP96/196
9240598 Brake Cylinder, AP96/196
9240588 Vacuum Switch Assy, Capper, AP96
9240578 Vacuum Switch Assy, Elevator, AP96
9240563 Fuse,PCB, 630, Eppendorf, AP196/98
9240526 Cable, Motor Axis #3, Capper I/O, AP98
9240500 Fluidic Valve, Reagent Pump, AP
9240240 Collet nut, TR
9240099 #Re-Installation, BR M48 The Re-Installation, BR M48 facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware and software check of instrument and provides user with operator refesher overview. Re-installation includes labor and travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.
9239855 Compressor, v2.0
9019494 Measurement Gauge Kit, RCS
9238863 LiquiChip, Premium Agreement The Premium Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).
5000-1251 Motor Control PCB, DML2
5000-1513 H-Spring, DML2
6000-3119 Plate Washer I - Rinse Cap Assembly
5000-1417 Overlay 099A, VTX2
5050-1078 LIS Information Package
9240717 Lead Screw Assy, Axis 5, TR, AP
9240703 O-Ring, Valve, Reagent Pump, AP
9020806 Rotor Timing Calibration Dye, RGQ
338134 Microbial DNA Positive Control
288105 bactotype Mycoplasma Mg/Ms PCR Kit (96)
275002 flocktype NDV Ab (2)
285515 cador C. burnetii PCR Reagent (96)
285115 cador TKP PCR Reagent (96)
9001901 #QIAgility (PC) Priority QIAgility (PC) Priority includes the QIAgility instrument, installation and extended warranty. Installation covers hardware and software installation of system and provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor and travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation and service visits are documented on a GMP/GLP-compliant Field Service documents.
800331 PCR Amplification
9019489 ZLV PLATE ADAPTOR BIOHIT
9019544 ZLV Skirt, low raw white 2427, CASx
9019580 ZLV Reed Switch assembly, C34
9020583 X-Belt, DML3000 185 Teeth
9020592 Optical Head Assembly, DML 3000
9021015 Encoder, Z Axis, VR, DCU
9013209 Cabling kit, 5V/24V, BR8000
9013189 Wheel, retractable tubing, dispenser, BR
9011757 Reagent holder, 1-trough 20 ml Holder for accommodating a single disposable trough (20 ml) on the worktable; must be used with the reagent holder tray
132632 NeXtal Stock Potassium formate (50) 10M Potassium formate - 50mL (4 x 12,5 ml tube)
761125 PAXgene Blood DNA Tube (100) 100 PAXgene Blood DNA Tubes