中文名稱(chēng)：Minibrain 抗體
英文名稱(chēng)：Anti-Minibrain
產(chǎn)品編號(hào)：BYK-10483R
別名：MNB_DROME; Serine/threonine-protein kinase minibrain; mnb; Dmel_CG42273; Dmel_CG7826; Dmel_CG7835; CG42273; CG7826; CG7835; Dm1; Dmel\CG42273; Dyrk1; DYRK1; ME-IV; min; Mnb; MNB.
產(chǎn)品介紹： This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格：0.1ml/0.2ml
濃度：1mg/1ml
抗體來(lái)源：本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類(lèi)型：兔來(lái)源為多抗，鼠來(lái)源為單抗
產(chǎn)品類(lèi)型：一抗
Minibrain抗體-Minibrain 抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融，保質(zhì)期一年。
Minibrain抗體-Minibrain 抗體免疫組化問(wèn)題解答
1、染色過(guò)強(qiáng)？
原 因：抗體濃度過(guò)高或孵育時(shí)間過(guò)長(zhǎng)
解決方法：降低抗體滴度、抗體孵育時(shí)間：室溫1小時(shí)或4度過(guò)夜
原 因：孵育溫度過(guò)高超過(guò)37度
解決方法：一般在室溫20-28度
原 因：DAB顯色時(shí)間過(guò)長(zhǎng)或濃度過(guò)高
解決方法：顯色時(shí)間不超過(guò)5-12分鐘，以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色？
原 因：操作過(guò)程中沖洗不充分
解決方法：每步?jīng)_洗3次每次5分鐘
原 因：組織中含過(guò)氧化物酶未阻斷
解決方法：可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)
原 因：組織中含內(nèi)原性*
解決方法：正常非免疫動(dòng)物血清再封閉
原 因：血清蛋白封閉不充分
解決方法：血清蛋白封閉不充分
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