人11-β-羥基類固醇脫氫酶2檢測(cè)試劑盒定量檢測(cè),具有靈敏性高,特異性強(qiáng),重復(fù)性好,性價(jià)比高,線性好;操作簡(jiǎn)單,精確度高;售前售后完善,隨時(shí)來實(shí)驗(yàn)室現(xiàn)場(chǎng)觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡(jiǎn)稱:Human 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) ELISA Kit
人11-β-羥基類固醇脫氫酶2檢測(cè)試劑盒注意事項(xiàng)以下四點(diǎn):
1,封閉:以下包以后是無關(guān)的蛋白質(zhì)溶液濃度高,涂層技術(shù)。隨函附上使許多不相關(guān)的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進(jìn)程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報(bào)價(jià)相對(duì)低廉,人11-β-羥基類固醇脫氫酶2檢測(cè)試劑盒可用于高濃度(5%~10%);和一些稀有的運(yùn)用各種動(dòng)物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗(yàn)的詳細(xì)實(shí)習(xí)。
2,洗板:能夠說,在中操作,清洗是在首要的關(guān)鍵技術(shù)。因?yàn)榫郾揭蚁┑人芰蠈?duì)蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號(hào)的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應(yīng)將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會(huì)有必定的誤差,非常人的要素(當(dāng)然也有洗衣機(jī)除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,人11-β-羥基類固醇脫氫酶2檢測(cè)試劑盒加抗體(抗標(biāo)本和兩個(gè)):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個(gè)電阻,但也要留意兩個(gè)反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當(dāng)?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽(yáng)空三操控,如剖析疑問的呈現(xiàn)。YB-PKBb-Hu 人蛋白激酶Bβ(PKBβ) ELISA Kit Human Protein Kinase B Beta (PKBb) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.132ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PKBb-Ra 大鼠蛋白激酶Bβ(PKBβ) ELISA Kit Rat Protein Kinase B Beta (PKBb) ELISA Kit Rattus norvegicus Rat 0.312-20ng/mL 0.114ng/mL sandwich 96T/48T 997.5/698.3 12 months
YB-PKBg-Hu 人蛋白激酶Bγ(PKBγ) ELISA Kit Human Protein Kinase B Gamma (PKBg) ELISA Kit Homo sapiens human 1.56-100ng/mL 0.56ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PK-c 犬丙酮酸激酶(PK) ELISA Kit Canine Pyruvate Kinase, Liver And RBC (PK) ELISA Kit Canis familiaris; Canine Dog 3.125-200U/L 1.6U/L sandwich 96T/48T 995/696.5 12 months
YB-PKCa-Hu 人蛋白激酶Cα(PKCα) ELISA Kit Human Protein Kinase C Alpha (PKCa) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.054ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PKCa-Mu 小鼠蛋白激酶Cα(PKCα) ELISA Kit Mouse Protein Kinase C Alpha (PKCa) ELISA Kit Mus musculus mouse 0.781-50ng/mL 0.31ng/mL sandwich 96T/48T 950/665 12 months
YB-PKCa-Ra 大鼠蛋白激酶Cα(PKCα) ELISA Kit Rat Protein Kinase C Alpha (PKCa) ELISA Kit Rattus norvegicus Rat 0.625-40ng/mL 0.237ng/mL sandwich 96T/48T 997.5/698.3 12 months
YB-PKCb1-Hu 人蛋白激酶Cβ1(PKCβ1) ELISA Kit Human Protein Kinase C Beta 1 (PKCb1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.06ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PKCb1-Ra 大鼠蛋白激酶Cβ1(PKCβ1) ELISA Kit Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.057ng/mL sandwich 96T/48T 997.5/698.3 12 months
YB-PKCd-Hu 人蛋白激酶Cδ(PKCδ) ELISA Kit Human Protein Kinase C Delta (PKCd) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.059ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PKCd-Mu 小鼠蛋白激酶Cδ(PKCδ) ELISA Kit Mouse Protein Kinase C Delta (PKCd) ELISA Kit Mus musculus mouse 1.56-100ng/mL 0.54ng/mL sandwich 96T/48T 905/633.5 12 months
YB-PKCd-Ra 大鼠蛋白激酶Cδ(PKCδ) ELISA Kit Rat Protein Kinase C Delta (PKCd) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.053ng/mL sandwich 96T/48T 950/665 12 months
YB-PKCe-Hu 人蛋白激酶Cε(PKCε) ELISA Kit Human Protein Kinase C Epsilon (PKCe) ELISA Kit Homo sapiens human 1.56-100ng/mL 0.58ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PKCe-Mu 小鼠蛋白激酶Cε(PKCε) ELISA Kit