詳細介紹
概述
在實際的蛋白分離、分析工作中，廣大科研工作者經(jīng)常會遇到某些疏水性蛋白及聚合物改性蛋白在常規(guī)SEC柱上出峰狀態(tài)與理論不符的情況。針對此種情況，賽分科技在SRT系列色譜柱的基礎上，專門推出針對該類樣品的Sepax SRT-C SEC系列色譜柱。該鍵合固定相采用的表面修飾技術，通過在高純度的硅膠基質上鍵合均勻的納米厚度中性親水涂層而制備得到。由于采用可控的化學修飾技術，確保了填料批間有著可靠的重現(xiàn)性。SEC填料采用化學鍵合技術，表面親水涂層覆蓋*，因此不僅具有優(yōu)異的穩(wěn)定性，而且對蛋白等生物樣品的非特異性吸附作用也非常小。同時，大的孔體積保證了高的載樣量及優(yōu)異的分辨率。
技術參數(shù)：
| 固定相 | SRT-C SEC-150 | SRT-C SEC-300 | SRT-C SEC- 500 |
| 材料 | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica |
| 顆粒大小 | 5 mm | 5 mm | 5 mm |
| 孔徑 (Å) | ~ 150 | ~ 300 | ~ 500 |
| 蛋白質分子量范圍 | 500 - 150,000 | 5,000 – 1,250,000 | 15,000 – 5,000,000 |
| 水溶性聚合物分子量范圍 | 500-25,000 | 1,000-100,000 | 25,000-500,000 |
| pH 穩(wěn)定性 | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) |
| 反壓(psi for a 7.8x300 mm) | ~ 700 | ~ 700 | ~ 700 |
| 壓力 (psi) | ~ 4,500 | ~ 3,500 | ~ 3,000 |
| 鹽濃度范圍 | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M |
| 使用溫度 (oC) | ~ 80 | ~ 80 | ~ 80 |
| 流動相兼容性 | Aqueous and organic | Aqueous and organic | Aqueous and organic |
訂購信息
Sepax SRT-C SEC-150
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT-C SEC-150 | 5um | 150 Å | 21.5x600mm | 235150-21560 |
| SRT-C SEC-150 | 5um | 150 Å | 21.5x300mm | 235150-21530 |
| SRT-C SEC-150 | 5um | 150 Å | 7.5x600mm | 235150-7560 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x300mm | 235150-7830 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x150mm | 235150-7815 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x50 (Guard) | 235150-7805 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x300mm | 235150-4630 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x150mm | 235150-4615 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x50 (Guard) | 235150-4605 |
Sepax SRT-C SEC-300
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT-C SEC-300 | 5um | 300 Å | 21.5x600mm | 235300-21560 |
| SRT-C SEC-300 | 5um | 300 Å | 21.5x300mm | 235300-21530 |
| SRT-C SEC-300 | 5um | 300 Å | 7.5x600mm | 235300-7560 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x300mm | 235300-7830 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x150mm | 235300-7815 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x50 (Guard) | 235300-7805 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x300mm | 235300-4630 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x150mm | 235300-4615 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x50 (Guard) | 235300-4605 |
Sepax SRT-C SEC-500
| 產(chǎn)品名稱 | 粒度 | 孔徑 | 規(guī)格 | 訂貨號 |
| SRT-C SEC-500 | 5um | 500 Å | 21.5x600mm | 235500-21560 |
| SRT-C SEC-500 | 5um | 500 Å | 21.5x300mm | 235500-21530 |
| SRT-C SEC-500 | 5um | 500 Å | 7.5x600mm | 235500-7560 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x300mm | 235500-7830 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x150mm | 235500-7815 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x50 (Guard) | 235500-7805 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x300mm | 235500-4630 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x150mm | 235500-4615 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x50 (Guard) | 235500-4605 |
注：以上為常備規(guī)格訂貨信息，其它規(guī)格或特殊定制產(chǎn)品，電詢。
