大鼠半胱氨酸蛋白酶抑制劑C（CysC）檢測試劑盒齊一生物科技（上海）有限公司以真誠、主動、周到、規(guī)范、熱情的服務(wù)很快得到廣大客戶的*好評。公司秉承“適應(yīng)多樣化的需求，提供專業(yè)化的服務(wù)"的經(jīng)營理念，為客戶提供*的服務(wù)。歡迎社會各界朋友前來洽談業(yè)務(wù)，共創(chuàng)輝煌

ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個樣品根據(jù)自己的數(shù)量來定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
大鼠半胱氨酸蛋白酶抑制劑C（CysC）檢測試劑盒每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測定結(jié)果.
在450nm波長處測定各孔的OD值.
9019649    Fuse kit (6.3Ax10, 5Ax5), PyroQ96    
9019602    Home flag, Y-axis, Cx8    
9019599    Housing, filter seal, CX1    
9019585    Slideout electrical tray, QGY    
9019547    Fan guard and filter, Cxx    
9019532    Tip ejector, QGY    
204163    QuantiTect SYBR Green PCR +UNG Kit (200)    The supplied, ready-to-use master mix contains SYBR Green I dye, ROX passive reference dye, HotStarTaq DNA Polymerase, and a dNTP mix in an optimized buffer. The dNTP mix includes dUTP, allowing optional treatment with UNG. The UNG enzyme is provided separay. For convenience, the master mix can be stored at 2–8°C.
204141    QuantiTect SYBR Green PCR Kit (40)    "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect SYBR Green PCR Master Mix, 2.0 ml RNase-free water

For quantitative, real-time PCR and two-step RT-PCR using SYBR Green detection."
9019428    Sensor, source home, RG3    
9019397    Belt, toothed, rotor drive, RG3    
9019350    Heater assembly, element, sensors, RG6    
1027415    FlexiTube siRNA 1 nmol    
9019336    Connector, JP5, Hi, Med, Lo, RG6    
9019335    Transistor, power PCB, heater supply, RG    
9234678    Reader Power Supply Board    
1027280    AllStars Negative Control siRNA (5 nmol)    
1027271    miScript Negative Control Inhibitor(5)    
1022076    Control (non-sil.) siRNA (5 nm)    
9019225    Valve - Release, PyroQ24    
9019223    Power Supply - Cooling, PyroQ24    
9019221    Cooling module, PyroQ24    
1061063    CT PCR REAGENT II    
1061064    CT Quantification Standards    
1061103    HCMV PCR REAGENT I    
1061105    MP PCR REAGENT II    
1061110    NG PCR REAGENT I    
1061123    HBV PCR REAGENT V    
9016386    EZ1 VRS Card    
9016234    BGR Zentrieradapter    
9019888    Lid, label 2CH, RG3    
9019877    Cover, main, blue, RG6    
9020805    Single LED, blue, Qsym    
9013538    Underlay, cooling block    
9013407    Tubing set, without PVC tubing, BR9604    
9238802    Sealing cap, clear, incl. clips    
9019625    PCB, level sensor rev8, Cx2    
9019579    LED, power assembly, C1x    
9019558    Pulley,  X idler, Al15T2.5/40-2, Cxx    
9019545    Cable, RS232, 9 pin, 2m, Cxx    
9019540    Kit, screws & washers, inst. covers, Cxx    
9019512    Tube, pinch valve, CX1    
9019503    TANK LOWER BUFFER ASSY GS    
217061    miRNeasy 96 Kit (4)    
204257    QuantiFast Probe PCR Kit (4000)    
9019391    Fan, cooler blower, RG3    
9019384    Board, main, dual & OEM, RG3    
9019356    Sensor, rotor home, RG6    
9019338    LED, red (625), RG6    
9019332    PCB, Power w/regulator trans, RG6    
9019312    Solenoid, door lock, EZ1xl    
9019235    Linear guide - Y, PyroQ24    
9234680    Rack for Reagents, Plastic block 40 h    
9020476    Fan, GSC    
9233430    Belt, tooth, Z-drive, BR8000    
9019198    Damper kit - Top cover, PyroQ96MD    
9019190    Bellows, PyroQ96MD    
31143    Pierce(R) Rabbit Anti-Human   IgG (H+L)    2.0 MG
31146    Pierce(R) Human IgM (Myeloma), Whole Molecule, 2.0 mg    2 MG
31148    Pierce(R) Human IgA Serum,    2.0 mg    2 MG
31154    Pierce(R) Human IgG, Whole    Molecule, 10 mg.    10 MG
31160    Pierce(R) Goat Anti-Mouse IgG (H+L), 2 mg    2 MG
31163    Pierce(R) F(ab')2 Fragment    Goat Anti-Human IgG (Fc)    1.0 MG
31164    Pierce(R) GOAT ANTI-MOUSE IgG  (H+L)(min X HnBvHs Sr PROT),    1.5 MG
31165    Pierce(R) F(ab')2 Fragment    Goat Anti-Human IgG (H+L)    1.0 MG
31166    Pierce(R) Goat Anti-Mouse IgG F(ab')2, 2 mg    2 MG
31168    Pierce(R) Goat Anti-Mouse IgG Fc, 2 mg    2 MG
31170    Pierce(R) Goat Anti-Mouse IgG Fc (min X HnBvHs Sr PROT), 1.5    1.5 MG
31172    Pierce(R) GOAT ANTI-MOUSE IgM u, 2 MG    2 MG
31178    Pierce(R) F(ab')2 FRAGMENT GO AT ANTI-MOUSE IgMu, 1 MG    1 MG
31181    Pierce(R) Horse Anti-Mouse Ig G (H+L)    1.5 MG
31182    Pierce(R) GOAT ANTI-MOUSE IgG +IgM (H+L), 2 MG    2 MG
31185    Pierce(R) F(ab')2 Fragment    Goat Anti-Mouse IgG (H+L)    1.0 MG
31186    Pierce(R) F(ab')2 Fragment    Goat Anti-Mouse IgM (u)    1.0 MG
31188    Pierce(R) Rabbit Anti-Mouse,  IgG (H+L), 2 mg    2 MG
31190    Pierce(R) Rabbit Anti-Mouse   IgG(H+L)(min X Hn Sr PROT),    1.5 MG
31192    Pierce(R) Rabbit Anti-Mouse IgG F(ab')2, 2 MG    2 MG
31194    Pierce(R) Rabbit Anti-Mouse   IgG Fc, 2 mg    2 MG
31196    Pierce(R) Rabbit Anti-Mouse IgMu, 2 MG    2 MG
31198    Pierce(R) Rabbit Anti-Mouse IgG+IgM (H+L), 2 MG    2 MG
31203    Pierce(R) Mouse IgG, F(ab')2  Fragment    2 MG
31205    Pierce(R) Mouse IgG, FC Fragm ent    1 MG
31210    Pierce(R) Goat Anti-Rabbit    IgG  (H+L), 2 mg    2 MG
31212    Pierce(R) Goat Anti-Rabbit IgG (H+L)(min X Hn Sr PROT), 1.5    1.5 MG
31213    Pierce(R) Mouse Anti-Rabbit   IgG (H+L), (min x GtHnMsSh Sr    1.5 MG
31216    Pierce(R) Goat Anti-Rabbit IgG Fc, 2 MG    2 MG
31218    Pierce(R) Rabbit Anti-Rat     IgG (H+L)    2.0 MG
31219    Pierce(R) Rabbit Anti-Rat IgG  (H+L), (min x Ms Sr Prot)    0.5 MG
31220    Pierce(R) Goat Anti-Rat IgG   (H+L), 2 mg    2 MG
31226    Pierce(R) Goat Anti-Rat IgG Fc,  2 MG    2 MG
31232    ImmunoPure (R) Goat Anti-Mouse IgG Fc gamma fragment    1 MG
31234    Pierce(R) Goat Anti-Rabbit IgG {F(ab')2}    2.0 MG
31235    Pierce(R) Rabbit IgG, Whole   Molecule, 10 mg    10 MG
31236    ImmunoPure (R) Goat Anti-Mouse IgG Fc gamma Subclass 1 (min    1 MG
31237    ImmunoPure (R) Goat Anti-Mouse IgG Fc gamma Subclass 2a (min    1 MG
31238    ImmunoPure (R) Donkey Anti-Rabbit IgG (H+L) (min x BvChGtGuH    1 MG
31239    ImmunoPure (R) F(ab')2        Fragment Goat Anti-Rabbit IgG    1 MG
31240    Pierce(R) Rabbit Anti-Sheep IgG (H+L), 2 MG    2 MG
31242    ImmunoPure (R) Mouse          Monoclonal Anti-Fluorescein    1 MG
31243    Pierce(R) Sheep IgG, Whole Molecule, 10 mg    10 MG
31245    Goat IgG, Whole Molecule,     10 mg.    10 MG
31246    Hamster IgG, Whole Molecule,  5 mg.    5 MG
31300    Pierce(R) Phosphatase Conjugated Rabbit Anti-Goat IgG (H+L),    1 ML
31302    Pierce(R) Phosphatase Conjugated F(ab')2 Fragment Rabbit Ant    0.5 ML
31310    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgG (H+L),    1 ML
31312    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgG F(ab')    1 ML
31314    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgAa, 1 mL    1 ML
31316    Pierce(R) Phosphatase Conjugated Goat Anti-Human IgA+IgG+IgM    1 ML
31318    Pierce(R) Phosphatase Conjugated Rabbit Anti-Human IgG (Fc)    1.0 ML
31320    Pierce(R) Goat Anti-Mouse IgG,(H&L), Alkaline Phosphatase    1 ML
31321    Pierce Goat Anti-Mouse IgG    (H+L) Alkaline Phosphatase    0.2 MG
31322    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG (H+L),    1 ML
31324    Pierce(R) Phosphatase Conjugated Goat Anti-Mouse IgG F(ab')    1 ML