人B-淋巴細(xì)胞趨化因子1(BLC-1/CXCL13)ELISA試劑盒

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人B-淋巴細(xì)胞趨化因子1(BLC-1/CXCL13)ELISA試劑盒

產(chǎn)品名稱：人水痘帶狀皰疹病毒IgG(VZV-IgG)Elisa   Kit。
規(guī)    格：96T/48T
品牌：進(jìn)口/國產(chǎn)
檢測標(biāo)本：血清，血漿，尿液，胸腹水，腦脊液，細(xì)胞培養(yǎng)上清，組織勻漿等
檢測方法：ELISA酶聯(lián)免疫夾心法
運(yùn)輸保存：2~8°保存6個月
特點(diǎn)：靈敏性高、特異性強(qiáng)、重復(fù)性好
服務(wù)：齊一生物所有的elisa試劑盒均免費(fèi)代測，全程Elisa實(shí)驗(yàn)技術(shù)指導(dǎo)，免費(fèi)代做實(shí)驗(yàn)。
運(yùn)輸：全國各地快遞免費(fèi)送貨上門
咨詢：    : 胡

人B-淋巴細(xì)胞趨化因子1(BLC-1/CXCL13)ELISA試劑盒

Drug Names
Generic Name：Human B-Lymphocyte Chemoattractant 1,BLC-1 ELISA Kit

Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：180ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃

Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 120ng/L,80ng/L ,40ng/L,20ng/L,10ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.

Calculate

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density,

Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

人B-淋巴細(xì)胞趨化因子1(BLC-1/CXCL13)ELISA試劑盒

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大鼠大內(nèi)皮素(Big ET)ELISA試劑盒    Rat Big Endothelin,Big ET ELISA試劑盒
大鼠大內(nèi)皮素(Big ET)ELISA試劑盒    Rat Big Endothelin,Big ET ELISA試劑盒
大鼠遲現(xiàn)抗原(VLA)ELISA試劑盒    Rat very late appearing antigen,VLA ELISA試劑盒
大鼠降鈣素(CT)ELISA試劑盒    Rat Calcitonin,CT ELISA試劑盒
大鼠骨鈣素/骨谷氨酸蛋白(OT/BGP)ELISA試劑盒    Rat Osteocalcin/Bone gla protein,OT/BGP ELISA試劑盒
大鼠可溶性核因子κB受體活化因子配基(sRANKL)ELISA試劑盒    Rat soluble receptor activator of nuclear factor-kB ligand,sRANKL ELISA試劑盒
大鼠促甲狀腺素(TSH)ELISA試劑盒     Rat Thyroid Stimulating Hormone,TSH ELISA試劑盒
大鼠(Cortisol)ELISA試劑盒    Rat Cortisol ELISA試劑盒
大鼠雌二醇(E2)ELISA試劑盒    Rat Estradiol,E2 ELISA試劑盒
大鼠脫氫表雄酮硫酸酯(DHEA-S)ELISA試劑盒    Rat Dehydroepiandrosterone sulfate,DHEA-S ELISA試劑盒
大鼠睪酮(T)ELISA試劑盒    Rat Testoterone,T ELISA試劑盒
大鼠游離睪酮(F-TESTO)ELISA試劑盒    Rat Free Testoterone,F-TESTO ELISA試劑盒
大鼠雄烯二酮(ASD)ELISA試劑盒    Rat Androstenedione,ASD ELISA試劑盒
大鼠雌三醇(E3)ELISA試劑盒    Rat Estriol,E3 ELISA試劑盒
大鼠17羥孕酮(17-OHP)ELISA試劑盒    Rat 17-Hydroxyprogesterone,17-OHP ELISA試劑盒
大鼠三碘甲狀腺原氨酸(T3)ELISA試劑盒    Rat Tri-iodothyronine,T3 ELISA試劑盒 
大鼠甲狀腺素(T4)ELISA試劑盒    Rat thyroxine,T4 ELISA試劑盒
大鼠游離甲狀腺素(FT4)ELISA試劑盒    Rat Free Thyroxine,FT4 ELISA試劑盒
大鼠游離三碘甲狀腺原氨酸(Free-T3)ELISA試劑盒    Rat Free Tri-iodothyronine Indes,Free-T3 ELISA試劑盒
大鼠新生甲狀腺素(NN-T4)ELISA試劑盒    Rat Neonatal Thyroxine,NN-T4 ELISA試劑盒
大鼠胰島素(INS)ELISA試劑盒    Rat Insulin,INS ELISA試劑盒
大鼠C肽(C-Peptide)ELISA試劑盒    Rat C-Peptide ELISA試劑盒
大鼠絨毛膜促性腺激素(CG)ELISA試劑盒    Rat Chorionic Gonadotrophin,CG ELISA試劑盒
大鼠促黃體激素(LH)ELISA試劑盒    Rat luteotropic hormone,LH ELISA試劑盒
大鼠抗心肌抗體(AMA)ELISA試劑盒     Rat Anti-myocardial antibody,AMA ELISA試劑盒
大鼠抗中性粒/中心體抗體(ACA)ELISA試劑盒    Rat anti-centrol and centrosome antibody,ACA ELISA試劑盒
大鼠磷脂酰肌醇抗體IgG/IgM(PI Ab-IgG/IgM)ELISA試劑盒    Rat phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA試劑盒
大鼠復(fù)合前列腺特異性抗原(CPSA)ELISA試劑盒    Rat complex edprostate specific antigen,cPSA ELISA試劑盒
大鼠胰蛋白酶(trypsin)ELISA試劑盒     Rat trypsin ELISA試劑盒
大鼠β干擾素(IFN-β/IFNB)ELISA試劑盒     Rat Interferon β,IFN-β/IFNB ELISA試劑盒
大鼠血小板因子3(PF-3)ELISA試劑盒     Rat plaet factor 3, PF3 ELISA試劑盒
大鼠可溶性瘦素受體(sLR)ELISA試劑盒    Rat Leptin Soluble Receptor,sLR ELISA試劑盒
大鼠窖蛋白Caveolin1(Cav-1)ELISA試劑盒    Rat Caveolin-1,Cav-1 ELISA試劑盒
大鼠艾杜糖硫酸酯酶(IDS)ELISA試劑盒     Rat iduronate sulfatase,IDS ELISA試劑盒
大鼠血管內(nèi)皮細(xì)胞生長因子(VEGF)ELISA試劑盒     Rat Vascular Endothelial cell Growth Factor,VEGF ELISA試劑盒
大鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒     Rat Tumor necrosis factor α,TNF-α ELISA試劑盒
大鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒     Rat Transforming Growth factor β1,TGF-β1 ELISA試劑盒 
大鼠干細(xì)胞因子/肥大細(xì)胞生長因子(SCF/MGF)ELISA試劑盒    Rat Stem cell factor/mast cell growth factor,SCF/MGF ELISA試劑盒
大鼠腺嘌呤二核苷酸磷酸(NADPH)ELISA試劑盒    Rat nicotinamide adenine dinucleotide phosphate,NADPH ELISA試劑盒
大鼠血小板衍生生長因子AB(PDGF-AB)ELISA試劑盒    Rat Plaet-Derived Growth Factor AB,PDGF-AB ELISA試劑盒
大鼠血小板衍生生長因子可溶性受體α(PDGFsR-α)ELISA試劑盒    Rat Plaet-Derived Growth Factor Soluble Receptor α,PDGFsR-α ELISA試劑盒
大鼠神經(jīng)營養(yǎng)因子4(NT-4)ELISA試劑盒    Rat Neurotrophin 4,NT-4 ELISA試劑盒
大鼠神經(jīng)營養(yǎng)因子3(NT-3)ELISA試劑盒    Rat Neurotrophin 3,NT-3 ELISA試劑盒 
大鼠神經(jīng)生長因子(NGF)ELISA試劑盒    Rat Nerve growth factor,NGF ELISA試劑盒
大鼠巨噬細(xì)胞炎性蛋白3α(MIP-3α/CCL20)ELISA試劑盒    Rat macrophage inflammatory protein 3α,MIP-3α ELISA試劑盒
大鼠層連蛋白/板層素(LN)ELISA試劑盒    Rat Laminin,LN ELISA試劑盒
大鼠白介素6(IL-6)ELISA試劑盒     Rat Interleukin 6,IL-6 ELISA試劑盒
大鼠白介素4(IL-4)ELISA試劑盒    Rat Interleukin 4,IL-4 ELISA試劑盒
大鼠白介素2(IL-2)ELISA試劑盒     Rat Interleukin 2,IL-2 ELISA試劑盒
大鼠白介素1α(IL-1α)ELISA試劑盒     Rat Interleukin 1α ,IL-1α ELISA試劑盒
大鼠白介素18(IL-18)ELISA試劑盒    Rat Interleukin 18,IL-18 ELISA試劑盒
大鼠白介素10(IL-10)ELISA試劑盒     Rat Interleukin 10,IL-10 ELISA試劑盒
大鼠胰島素樣生長因子2(IGF-2)ELISA試劑盒    Rat Insulin-like growth factor 2,IGF-2 ELISA試劑盒
大鼠胰島素樣生長因子1(IGF-1)ELISA試劑盒    Rat Insulin-like growth factor 1,IGF-1 ELISA試劑盒
大鼠γ干擾素(IFN-γ)ELISA試劑盒    Rat Interferon γ ,IFN-γ ELISA試劑盒
大鼠細(xì)胞間粘附分子1(ICAM-1/CD54)ELISA試劑盒     Rat intercellular adhesion molecule 1,ICAM-1 ELISA試劑盒
大鼠粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)ELISA試劑盒    Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA試劑盒
大鼠膠質(zhì)細(xì)胞系來源的神經(jīng)營養(yǎng)因子(GDNF)ELISA試劑盒    Rat glial cell line-derived neurotrophic factor,GDNF ELISA試劑盒
大鼠趨化因子(fractalkine/CX3CL1)ELISA試劑盒    Rat Fractalkine/CX3CL1 ELISA試劑盒
大鼠纖連蛋白(FN)ELISA試劑盒     Rat Fibronectin,FN ELISA試劑盒
大鼠睫狀神經(jīng)營養(yǎng)因子(CNTF)ELISA試劑盒    Rat Ciliary Neurotrophic Factor,CNTF ELISA試劑盒
大鼠骨成型蛋白受體Ⅱ(BMPR-Ⅱ)ELISA試劑盒    Rat Bone morphogenetic protein receptor Ⅱ,BMPR-Ⅱ ELISA試劑盒
大鼠骨成型蛋白受體1A(BMPR-1A)ELISA試劑盒    Rat Bone morphogenetic protein receptor 1A,BMPR-1A ELISA試劑盒
大鼠骨成型蛋白4(BMP-4)ELISA試劑盒    Rat Bone morphogenetic protein 4,BMP-4 ELISA試劑盒
大鼠骨成型蛋白4(BMP-4)ELISA試劑盒    Rat Bone morphogenetic protein 4,BMP-4 ELISA試劑盒
大鼠骨成型蛋白2(BMP-2)ELISA試劑盒    Rat Bone morphogenetic protein 2,BMP-2 ELISA試劑盒
大鼠腦源性神經(jīng)營養(yǎng)因子(BDNF)ELISA試劑盒    Rat Brain derived neurotrophic facor,BDNF ELISA試劑盒
大鼠活化素A(ACV-A)ELISA試劑盒    Rat Activin A,ACV-A ELISA試劑盒
大鼠血管緊張素Ⅱ(ANG-Ⅱ)ELISA試劑盒    Rat angiotension Ⅱ,ANG-Ⅱ ELISA試劑盒
大鼠血管緊張素轉(zhuǎn)化酶(ACE)ELISA試劑盒    Rat Angiotensin converting enzyme,ACE ELISA試劑盒
大鼠巨噬細(xì)胞炎癥蛋白1α(MIP-1α/CCL3)ELISA試劑盒    Rat macrophage inflammatory protein 1α,MIP-1α ELISA試劑盒
大鼠巨噬細(xì)胞炎癥蛋白1β(MIP-1β/CCL4)ELISA試劑盒     Rat macrophage inflammatory protein 1β,MIP-1β ELISA試劑盒
裸鼠環(huán)加氧酶2(COX-2)ELISA試劑盒    Nude mouse cyclooxygenase-2,COX-2 ELISA試劑盒
裸鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒     Nude mouse Alpha-fetoprotein,AFP ELISA試劑盒 
裸鼠克拉拉細(xì)胞蛋白(CC16) ELISA試劑盒    Nude Mouse Clara cell protein,CC16 ELISA試劑盒 
裸鼠蛋白磷酸酶(PP)ELISA試劑盒    Nude Mouse Protein Phosphatase,PP ELISA試劑盒 
倉鼠基質(zhì)金屬蛋白酶9/明膠酶B(MMP-9/Gelatinase B) ELISA試劑盒    Hamster matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA K
倉鼠核因子κB受體活化因子配基(RANKL)ELISA試劑盒    Hamster receptor activator of nuclear factor kappa B ligand,RANKL ELISA試劑盒 
倉鼠甲狀腺結(jié)合球蛋白(TBG)ELISA試劑盒    Hamster Thyroxine-Binding Globulin Assay,TBG ELISA試劑盒 
倉鼠組織蛋白酶K(cath-K)ELISA試劑盒    Hamster Cathepsin K,cath-K ELISA試劑盒 
豚鼠卵清蛋白特異性IgG(OVA sIgG)ELISA試劑盒     Guinea pig ovalbumin specific IgG,OVA sIgG ELISA試劑盒
豚鼠白介素4(IL-4)ELISA試劑盒     Guinea pig interleukin 4,IL-4 ELISA試劑盒 
豚鼠免疫球蛋白G(IgG)ELISA試劑盒    Guinea pig Immunoglobulin G,IgG ELISA試劑盒
豚鼠鉤端螺旋體IgG(Lebtospira)ELISA試劑盒    Guinea pig Lebtospira IgG ELISA試劑盒
豚鼠白三烯D4(LTD4)ELISA試劑盒     Guinea pig leukotriene D4,LT-D4 ELISA試劑盒 
豚鼠卵清蛋白特異性IgE(OVA sIgE)ELISA試劑盒    Guinea pig ovalbumin specific IgE,OVA sIgE ELISA試劑盒
豚鼠白介素12(IL-12/P70)ELISA試劑盒     Guinea pig Interleukin-12,IL-12/P70 ELISA試劑盒
豚鼠一氧化氮(NO)ELISA試劑盒    guinea pig nitric oxide,NO ELISA試劑盒
豚鼠主要組織相容性復(fù)合體(MHC/GPLA)ELISA試劑盒    Guinea pig major histocompatibility complex,MHC/GPLA ELISA試劑盒
豚鼠內(nèi)皮型一氧化氮合成酶(eNOS)ELISA試劑盒     Guinea Pig Endothelial nitric oxide synthase,eNOS ELISA試劑盒 
豚鼠降鈣素基因相關(guān)肽(CGRP)ELISA試劑盒    Guinea Pig calcitonin gene related peptide,CGRP ELISA試劑盒
豚鼠纖溶酶抗纖溶酶復(fù)合物(PAP)ELISA試劑盒    Guinea Pig plasmin-antiplasmin complex,PAP ELISA試劑盒
豚鼠血清總補(bǔ)體(CH50)ELISA試劑盒     Guinea Pig 50% complement hemolysis,CH50 ELISA試劑盒 
豚鼠超氧化物歧化酶(SOD)ELISA試劑盒    Guinea Pig Super Oxidase Dimutase,SOD ELISA試劑盒 
豚鼠組胺(HIS)ELISA試劑盒    guinea pig Histamine,HIS ELISA試劑盒
豚鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒     guinea pig transforming growth factor β1,TGF-β1 ELISA試劑盒 
豚鼠腫瘤壞死因子α(TNF-α)ELISA試劑盒    Guinea pig Tumor necrosis factor α,TNF-α ELISA試劑盒
豚鼠生長激素(GH)ELISA試劑盒     Guinea pig Growth hormone,GH ELISA試劑盒
豚鼠內(nèi)皮素1(ET-1)ELISA試劑盒    guinea pig Endothelin 1,ET-1 ELISA試劑盒
豚鼠免疫球蛋白E(IgE)ELISA試劑盒    guinea pig Immunoglobulin E,IgE ELISA試劑盒 
豚鼠免疫球蛋白A(IgA)ELISA試劑盒     guinea pig Immunoglobulin A,IgA ELISA試劑盒
豚鼠基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)ELISA試劑盒    guinea pig tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA試劑盒