兔Bcl-2相關(guān)X蛋白(BAX)ELISA試劑盒規(guī)    格：96T/48T
檢測標(biāo)本：血清，血漿，尿液，胸腹水，腦脊液，細(xì)胞培養(yǎng)上清，組織勻漿等
檢測的原理：試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗(yàn)（ELISA）
產(chǎn)品的用途：僅供科研課題使用
試劑盒保存：2-8℃。
有效期：6個(gè)月
運(yùn)輸方法：泡沫箱配生物冰袋運(yùn)輸兔Bcl-2相關(guān)X蛋白(BAX)ELISA試劑盒
價(jià)格及詳細(xì)資料：電議,或咨詢在線客服,或者以郵件形式發(fā)到我司qysw@qiyibio.com .齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大科研單位*肯定和認(rèn)同。*保證，價(jià)格公道，傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供產(chǎn)品。若有需要，我司將竭誠為您服務(wù)！
FOR RESEARCH USE ONLY
Drug Names
Generic Name：兔Bcl-2相關(guān)X蛋白(BAX)ELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

其它產(chǎn)品ABCG2 Antibody    Q9UNQ0    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB IHC ELISA
Abi-1 Antibody    Q8IZP0    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
ACBP Antibody    P07108    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB IHC ELISA
ACE1 Antibody    P12821    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
ACE2 Antibody    Q9BYF1    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M    WB ELISA
AChE Antibody    P22303    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
AChRα1 Antibody    P02708    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    IHC ELISA
AChRα3 Antibody    P32297    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
AChRα5 Antibody    P30532    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
ACK Antibody    Q07912    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, R    WB ELISA
Acrp30 Antibody    Q15848    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
ACTR-IIA Antibody    P27037    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
Adducin α Antibody    P35611    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
ADM Antibody    P35318    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    IHC ELISA
AID Antibody    Q9GZX7    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M    WB ELISA
Akt Antibody    P31749    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
ALDH3A1 Antibody    P30838    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, R    WB ELISA
ALK Antibody    Q9UM73    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M    IHC ELISA
ALK Antibody    Q9UM73    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB ELISA
Ameloblastin Antibody    Q9NP70    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB ELISA
Ang-1 Antibody    Q15389    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
Annexin I Antibody    P04083    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
Annexin II Antibody    P07355    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
Annexin III Antibody    P12429    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M    WB IHC ELISA
ApoE Antibody    P02649    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
AQP0 Antibody    P30301    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
AQP3 Antibody    Q92482    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    IHC ELISA
AQP4 Antibody    P55087    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
AQP5 Antibody    P55064    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB IHC ELISA
Arrestin-β-1 Antibody    P49407    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB ELISA
ASCL1 Antibody    P50553    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
ATP5J2 Antibody    A6ND55    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    IHC ELISA
ATP7A Antibody    Q04656    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    IHC ELISA
BMP-2 Antibody    P12643    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
BMP-3 Antibody    P12645    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
BMP-4 Antibody    P12644    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
BMP-5 Antibody    P22003    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M    WB IHC ELISA
BMP-6 Antibody    P22004    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    WB IHC ELISA
BMP-7 Antibody    P18075    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
BMP-8 Antibody    P34820/Q7Z5Y6    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H    IHC ELISA
BNIP-2 Antibody    Q12982    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M    WB IHC ELISA
BNIP-3 Antibody    Q12983    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB IHC ELISA
BRCAA1 Antibody    Q4LE39    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
Brg-1 Antibody    P51532    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
Brm Antibody    P51531    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, M, R    WB ELISA
CA II Antibody    P00918    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    H, R    WB IHC ELISA