HMGB1高遷移率族蛋白B1抗體英文名稱：HMGB1
中文名稱：高遷移率族蛋白B1抗體
別    名：Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; High mobility group protein B1; HMG3; HMGB 1;HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.
研究領域：腫瘤  細胞生物  免疫學  轉錄調節(jié)因子  結合蛋白  
抗體來源：Rabbit
克隆類型：Polyclonal
交叉反應：Human, Mouse, Rat, Cow, 
產品應用：WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量：24kDa
HMGB1高遷移率族蛋白B1抗體細胞定位：細胞核 
性    狀：Lyophilized or Liquid
濃    度：1mg/1ml
免 疫 原：KLH conjugated synthetic peptide derived from human HMGB1
亞    型：IgG
純化方法：affinity purified by Protein A
儲 存 液：0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

產品介紹：background:
High Mobility Group Box-1 (HMGB1) is a cytokine implicated in the pathogenesis of rheumatoid arthritis (RA) and other inflammatory diseases. The cholinergic anti-inflammatory pathway, a vagus nerve dependent mechanism, inhibits HMGB1 release in experimental disease models 

Function:
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).

Subunit:
Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2 (By similarity).

Subcellular Location:
Nucleus. Chromosome. 

Similarity:
Belongs to the HMGB family. 
Contains 2 HMG box DNA-binding domains.

Gene ID:
3146

Database links:
Entrez Gene: 282691 Cow
Entrez Gene: 3146 Human
Entrez Gene: 100862258 Mouse
Entrez Gene: 15289 Mouse
Entrez Gene: 25459 Rat
Omim: 163905 Human
SwissProt: P10103 Cow
SwissProt: P09429 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

近來的研究表明稱之為高遷移率族蛋白B-1（HMG-B1）的核內結構蛋白在核外表達時是一種有效的早期炎癥介質。
高遷移性Ｂ１組蛋白（ＨＭＧＢ１）： 是一種核結合蛋白，在ＤＮＡ重組、修復、復制和基因轉錄中起作用。ＨＭＧＢ１也是巨噬細胞分泌的一種介質。
此外，高遷移性Ｂ１組蛋白亦被受刺激的巨噬細胞或單核細胞主動分泌。在這一主動分泌過程中，HMGB1首先經(jīng)乙?；⒂珊藘绒D移至溶酶體內，繼而在ATP和溶血磷脂膽堿兩種分泌信號指導下轉移至細胞外。由壞死細胞被動釋放的HMGB1和炎癥細胞主動分泌的HMGB1存在分子上的差異。胞外的HMGB1可作為細胞因子參與信號傳導，因為它既可識別Toll 樣受體（TLR）家族的一些成員，又能與識別晚期糖基化終末產物受體（RAGE）相作用。
HMGB1能啟動炎癥反應，包括產生多種細胞因子、對某些干細胞產生趨化作用、誘導血管粘附分子、削弱腸上皮細胞的功能等。

Protein: line1,mo macrophages lysates, 30ug; 

Primary: Anti-HMGB1 (bs-0664R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000; 
ECL excitated the fluorescence; 
Predicted band size : 24kD
Observed band size : 24kD