豬圓環(huán)病毒Ⅱ型衣殼蛋白抗體capsid protein相關(guān)標(biāo)記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin  Cy3   Cy5  
 Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC      
英文名稱    capsid protein
中文名稱    豬圓環(huán)病毒Ⅱ型衣殼蛋白抗體
別    名    ORF1; ORF2 (PCV-2; PCV 2; Porcine circovirus2; Porcine circovirus-2.)
研究領(lǐng)域    細(xì)胞生物  免疫學(xué)  細(xì)菌及病毒  
抗體來源    Rabbit
克隆類型    Polyclonal
交叉反應(yīng)    PCV2
產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 

not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    26kDa
性    狀    Lyophilized or Liquid
濃    度    1mg/1ml
免 疫 原    KLH conjugated synthetic peptide derived from PCV2 capsid protein
亞    型    IgG
豬圓環(huán)病毒Ⅱ型衣殼蛋白抗體capsid protein純化方法    affinity purified by Protein A
儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed    PubMed
產(chǎn)品介紹    background:
There has been much uncertainty in the relationship between PCV2 and PMWS but Bolin et al demonstrated that Koch's postulates could be fulfilled by inoculating cesarean-derived, colostrum-deprived (CDCD) pigs with PCV2 and producing clinical signs of PMWS. Similar work has been performed using an infectious genomic clone of PCV2 in specific pathogen free (SPF) pigs to produce the gross and microscopic lymphoid lesions of PMWS. Although PCV2 has been shown to induce PMWS lesions in CDCD and SPF pigs, the presence of additional factors are required.

Function:
Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope.

Subunit:
Homomultimer. Assembles in the nucleus, presumably in an immature form, then migrates to the cytoplasm once assembled as mature virion (Probable). Interacts with Rep; this interaction relocates Rep into the nucleus.

Subcellular Location:
Host nucleus. Virion (Potential).

Similarity:
Belongs to the circoviridae capsid protein family.

Database links:
UniProtKB/Swiss-Prot: O56129.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

豬圓環(huán)病毒-PCV-2是一種嗜單核-巨噬細(xì)胞性的病毒，屬于圓環(huán)病毒科圓環(huán)病毒屬的成員，根據(jù)抗原性及基因組成，分為PCVl和PCV2兩種，其中PCV2對豬具有致病性，PCV2(病毒囊膜衣殼蛋白(Cap))主要侵害免疫系統(tǒng)，導(dǎo)致感染豬免疫抑制，降低機(jī)體對其他病原體的抵抗力和疫苗的反應(yīng)性，容易導(dǎo)致其他病毒和細(xì)菌的繼發(fā)感染，使死亡率明顯升高。