Rabbit Anti-CD163/FITC標(biāo)記抗體說明書
Catalog Number : RS-92527R-FITC
Quantity size :100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and
0.1% Sodium azide. Reconstitute with 100ul sterile distilled water. )
Backgroud:
CD163 is a 130 kDa membrane glycoprotein. It is a member of the scavenger receptor
cysteine-rich superfamily and is a receptor for the hemoglobin-haptoglobin complex. CD163 is
expressed exclusively on the cell surface of human monocytes and macrophages that evolve
predominantly in the late phase of inflammation. CD163 is present on all CD14 positive
monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive
monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and
dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce
shedding of CD163 from the cell surface into plasma or cell supernatant.
Function:
Acute phase-regulated receptor involved in clearance and endocytosis of
hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free
hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via
endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds
hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits
a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype
than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a
cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization,
inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher
capacity for ligand endocytosis and the more pronounced surface expression when expressed in
cells.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized
antibody is stable at room temperature for at least one month and for greater than a year
when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody,
the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in
human, therapeutic or diagnostic applications.