細(xì)胞名稱

HuH-7人肝癌細(xì)胞（干細(xì)胞庫保藏）

描述

人肝癌細(xì)胞

動(dòng)物種別

人

組織來源

肝臟

形態(tài)

上皮樣

培養(yǎng)基和添加劑

DMEM (Invitrogen, 12430) 88ml FBS (Biochrom, S0115) 10ml Glutamax (Invitrogen, 35050) 1ml Non-essential Amino Acids, 100X (Invitrogen, 11140) 1ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.Randall G,Grakoui A,Rice CM Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):235-40 Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.Blight KJ,McKeating JA,Rice CM J Virol. 2002 Dec;76(24):13001-14 Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.Lohmann V,K?rner F,Koch J,Herian U,Theilmann L,Bartenschlager R Science. 1999 Jul 2;285(5424):110-3 Establishment and characteristics of human hepatocellular carcinoma cells with metastasis to lymph nodes.Seki S,Kitada T,Kawada N,Sakaguchi H,Kadoya H,Nakatani K,Satake K,Kuroki T Hepatogastroenterology. 1999 Sep-Oct;46(29):2812-7 Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J Cancer Res. 1985 Dec;45(12 Pt 1):6379-83 Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.Nakabayashi H,Taketa K,Yamane T,Miyazaki M,Miyano K,Sato J Gann. 1984 Feb;75(2):151-8 Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J Cancer Res. 1982 Sep;42(9):3858-63