細(xì)胞名稱(chēng)

STO小鼠胚胎成纖維細(xì)胞STO（干細(xì)胞庫(kù)保藏）

描述

小鼠胚胎成纖維細(xì)胞，每支細(xì)胞量1×10⁶

動(dòng)物種別

小鼠，SIM品系

組織來(lái)源

胚胎

形態(tài)

成纖維細(xì)胞

培養(yǎng)基和添加劑

小鼠胚胎成纖維細(xì)胞STO*培養(yǎng)基配方（100 ml）： DMEM (Invitrogen, 12430) 88 ml FBS (Biochrom, S4115) 10 ml Glutamax(Invitrogen, 35050) 1 ml Non-essential Amino Acids, 100× (Invitrogen, 11140) 1 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416 Teratomas and Differentiation, eds. Michael I. Sherman and David Solter, 13–14. New York: Academic Press, 1975. Cell 6: 467-474, 1975. Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624 The line was derived from a continuous line of SIM mouse embryonic fibroblasts by A. Bernstein at the Ontario Cancer Institute.