細(xì)胞名稱

THP-1人單核細(xì)胞（2014年新引進(jìn)）（干細(xì)胞庫保藏）

描述

人單核細(xì)胞

動(dòng)物種別

人

性別

男

組織來源

外周血

形態(tài)

單核細(xì)胞

培養(yǎng)基和添加劑

人單核細(xì)胞THP-1*培養(yǎng)液配方（100 ml）： RPMI Medium 1640 (Invitrogen, 11875-093) 90 ml FBS (Biochrom, S0115) 10 ml ?-Mer (invitrogen 21985) 0.05mM 傳代周期：控制細(xì)胞密度在2?4 ? 105 cell / mL，按此比例進(jìn)行傳代 傳代比例：控制細(xì)胞密度在2?4 ? 105 cell / mL，按此比例進(jìn)行傳代 換液頻率：傳代時(shí)換液 凍存液：培養(yǎng)液95%，DMSO 5% 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。?

供應(yīng)限制

REFERENCE

Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641 Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed:9270019 Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed:8676475 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116 Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819 Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812 Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011 Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838 Tsuchiya S, et al. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int. J. Cancer 26: 171-176, 1980. PubMed: 6970727