細(xì)胞名稱

SV40 MES 13小鼠腎小球系膜細(xì)胞

描述

這株細(xì)胞的細(xì)胞骨架染色突出呈現(xiàn)肌動蛋白，在細(xì)胞質(zhì)中有豐富的平行微絲。 有報道稱在1uM血管緊張素II存在下，它們會收縮。 細(xì)胞在軟瓊脂中形成克隆，SV40大T抗原陽性。

動物種別

小鼠

性別

***

組織來源

腎小球系膜細(xì)胞

形態(tài)

心肌成纖維細(xì)胞樣

培養(yǎng)基和添加劑

DMEM培養(yǎng)基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，71.25%；F-12培養(yǎng)基(GIBCO,貨號21700075，添加L-glutamine 300mg/L, NaHCO3 1.5g/L)，23.75%；添加14mM HEPES；優(yōu)質(zhì)胎牛血清，5%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

22754: MacKay K, et al. Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice. Kidney Int. 33: 677-684, 1988. PubMed: 2835539 51029: Robey RB, et al. Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway. Am. J. Physiol. 277: F742-F749, 1999. PubMed: 10564237 51030: Robey RB, et al. Thrombin is a novel regulator of hexokinase activity in mesangial cells. Kidney Int. 57: 2308-2318, 2000. PubMed: 10844601 61211: Robey RB, et al. Regulation of mesangial cell hexokinase activity and expression by heparin-binding epidermal growth factor-like growth factor: epidermal growth factors and phorbol esters increase glucose metabolism via a common mechanism involving classic mitogen-activated protein kinase pathway activation and induction of hexokinase II expression. J. Biol. Chem. 277: 14370-14378, 2002. PubMed: 11782486 61224: Maile S, et al. The morphology of mesangial cells cultured at high density and in collagen gels. Histol. Histopathol. 15: 403-414, 2000. PubMed: 10809358 61225: Coy PE, et al. LPA is a novel lipid regulator of mesangial cell hexokinase activity and HKII isoform expression. Am. J. Physiol. Renal Physiol. 283: F271-F279, 2002. PubMed: 12110510 90657: Taneja N, et al. Proinflammatory interleukin-1 cytokines increase mesangial cell hexokinase activity and hexokinase II isoform abundance. Am. J. Physiol. Cell Physiol. 287: C548-C557, 2004. PubMed: 15070811