細(xì)胞名稱

AML12小鼠肝細(xì)胞AML12（干細(xì)胞庫(kù)保藏）

描述

小鼠肝實(shí)質(zhì)細(xì)胞，每支細(xì)胞量1×10⁶

動(dòng)物種別

小鼠，CD1品系

性別

雄

組織來源

3月齡的小鼠肝臟

形態(tài)

上皮細(xì)胞

培養(yǎng)基和添加劑

小鼠肝實(shí)質(zhì)細(xì)胞AML12*培養(yǎng)液配方（100 mL）： DMEM/F-12 (Invitrogen, 11330) 89 ml ITS Liquid Media Supplement (Sigma, I3146) 1 ml Dexamethasone 40 ng/ml FBS (Biochrom, S4115) 10 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Wu JC, et al. Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha. Proc. Natl. Acad. Sci. USA 91: 674-678, 1994. PubMed: 7904757 Dumenco L, et al. Introduction of a murine p53 mutation corresponding to human codon 249 into a murine hepatocyte cell line results in growth advantage, but not in transformation. Hepatology 22: 1279-1288, 1995. PubMed: 7557882