細(xì)胞名稱
G-Olig2小鼠胚胎干細(xì)胞G-Olig2(干細(xì)胞庫(kù)保藏)
描述
小鼠*性胚胎干細(xì)胞,每支細(xì)胞量1×106,帶有GFP基因,當(dāng)其分化成神經(jīng)細(xì)胞時(shí)GFP基因表達(dá)
動(dòng)物種別
小鼠,129X1/SvJ品系
組織來(lái)源
小鼠胚胎內(nèi)細(xì)胞團(tuán)
形態(tài)
球形克隆
培養(yǎng)基和添加劑
mES*培養(yǎng)液配方(600 ml): DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(終濃度為1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 氣相:空氣,95%;二氧化碳,5%。溫度:37攝氏度。
供應(yīng)限制
A。僅供研究之用。
REFERENCE
Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach, Oxford, Oxford University Press, 101-132, 1999 Xian HQ, et al. A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells 21: 41-49, 2003. PubMed: 12529550