好看日韩在线视频免费,日本不卡一区二区三区,三级a全过程在线观看,亚洲精品国产9999久久久久

上海酶聯(lián)生物研究所

當(dāng)前位置:首頁   >>   資料下載   >>   Human CH50

Human CH50

時間:2017/11/2閱讀:187
分享:
  • 提供商

    上海酶聯(lián)生物研究所
  • 資料大小

    73.5KB
  • 資料圖片

  • 下載次數(shù)

    23次
  • 資料類型

    WORD 文檔
  • 瀏覽次數(shù)

    187次
點(diǎn)擊免費(fèi)下載該資料

                          Human    CH50

FOR RESEARCH USE ONLY

Assay range:20 IU/ml -480 IU/ml                96 determinations
Purpose
For the quantitative in vitro determination of CH50 concentrations in Human serum, cell culture supernates and other biological fluidsPrinciple of the assay
The kit assay Human CH50 level in the sample,use Purified Human CH50 antibody to coat microtiter plate wells, make solid-phase antibody, then add CH50 to wells, Combined CH50 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human CH50 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit

Specimen requirements
1.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure

2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B 50ul to each well, evade the light preservation for 10 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.

calculate
Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.

Storage and validity
1.Storage:2-8℃.
2.validity: six months

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個,單個標(biāo)簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復(fù)您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),環(huán)保在線對此不承擔(dān)任何保證責(zé)任。

溫馨提示:為規(guī)避購買風(fēng)險,建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

在線留言
欧美精品啪啪人妻一区二区-嫩草人妻舔舔羞羞一区二区三区| 日韩中文字幕v亚洲中文字幕-日韩亚洲av免费在线观看| 国产福利视频一区二区三区-日韩人妻中文视频精品| 日韩二级视频在线观看-美女扒开奶罩露出奶子的视频网站| 欧美黄色三级视频网站-国产九九热视频在线观看| 黄色av网站在线免费观看-亚洲欧美精品偷拍tv| 日本高清二区视频久二区-大香蕉在线视频大香蕉在线视频| 青木玲高清中文字幕在线看-视频在线免费观看你懂的| 亚洲午夜久久久精品影院-性感美女在线观看网站国产| 91九色蝌蚪丝袜人妻-国产精品9999网站| 91大神国内精品免费网站-亚洲免费电影一区二区| 午夜精品午夜福利在线-内射无套内射国产精品视频| 在线成色中文综合网站-国产二区精品视频在线观看| 欧美日韩国产亚洲免费-肉体粗喘娇吟国产91| 国产午夜精品理论片A级漫画-久久精品国产99亚洲精品| 午夜福利卫生纸福利院-一区二区三区久久亚洲| 国产精品电影在线一区-亚洲国产大片一区二区官网| 免费av一区在线观看-国产精品视频高潮流白浆视频免费| 美女把腿张开给帅哥桶-无码无套少妇18p在线直播| 人妻日韩精品中文字幕图片-麻豆极度性感诱人在线露脸| 久久免费观看归女高潮特黄-黄色av一本二本在线观看| 国产精品二区高清在线-91精品91久久久久久| 亚洲精品蜜桃在线观看-国产欧美日韩在线观看精品观看| 欧美日韩精品人妻在线-在线播放中文字幕一区| 国产精品美女在线网址-久草免费福利在线观看视频| 哦啊好大用力欧美视频-麻豆国产传媒片在线观看| 熟女少妇免费一区二区-麻豆一区二区三区免费在线观看| 天天日天天干天天综合-99久久综合狠狠综合久久| 久久久噜噜噜久久狠狠50岁-精品一区二区三区av| 亚洲中文一二三av网-亚洲天堂成人免费在线| 麻豆久久国产精品亚洲-日本理论中文字幕在线视频| 中文字幕人妻少妇第一页-隔壁的女孩在线看中文字幕| 深夜三级福利在线播放-日韩精品一区二区在线天天狠天| 亚洲精品激情一区二区-久久成人国产欧美精品一区二区| 小12萝自慰喷水亚洲网站-chinese偷拍一区二区三区| 五月婷婷六月在线观看视频-亚洲黑寡妇黄色一级片| 欧美伦乱淫老妇女激情吧-亚洲女邻居精品二区久久| 午夜福利院免费在线观看-久久精品日产第一区二区三区画质| 天天干天天天天天天天-亚洲综合av在线三区| 免费午夜福利在线观看-黄色日本黄色日本韩国黄色| 国产精品熟女视频一区二区-国产日韩精品欧美一区喷水|