污水處理設(shè)備 污泥處理設(shè)備 水處理過濾器 軟化水設(shè)備/除鹽設(shè)備 純凈水設(shè)備 消毒設(shè)備|加藥設(shè)備 供水/儲水/集水/排水/輔助 水處理膜 過濾器濾芯 水處理濾料 水處理劑 水處理填料 其它水處理設(shè)備
上海極威生物科技有限公司
暫無信息 |
閱讀:163發(fā)布時間:2018-08-09
上海極威生物科技有限公司
236.7KB
18次
PDF 文件
163次
本試劑盒只能用于科學(xué)研究,不得用于醫(yī)學(xué)診斷
大鼠過氧亞硝基陰離子(ONOO-)ELISA 檢測試劑盒
使用說明書
檢測原理
試劑盒采用雙抗體夾心法酶聯(lián)免疫吸附試驗(yàn)(ELISA)。往預(yù)先
包被大鼠過氧亞硝基陰離子(ONOO-)捕獲抗體的包被微孔中,依次
加入標(biāo)本、標(biāo)準(zhǔn)品、HRP標(biāo)記的檢測抗體,經(jīng)過溫育并*洗滌。用
底物TMB顯色,TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作
用下轉(zhuǎn)化成終的黃色。顏色的深淺和樣品中的大鼠過氧亞硝基陰
離子(ONOO-)呈正相關(guān)。用酶標(biāo)儀在450nm 波長下測定吸光度(OD
值),計(jì)算樣品濃度。
樣品收集、處理及保存方法
5. 保存:如果樣本收集后不及時檢測,請按一次用量分裝,凍存
于-20℃,避免反復(fù)凍融,在室溫下解凍并確保樣品均勻地充分解凍。
自備物品
操作注意事項(xiàng)
存。
試劑盒組成
名稱 | 96 孔配置 | 48 孔配置 | 備注 |
|
|
|
|
微孔酶標(biāo)板 | 12 孔×8 條 | 12 孔×4 條 | 無 |
|
|
|
|
標(biāo)準(zhǔn)品 | 0.3mL | 0.3mL | 無 |
|
|
|
|
* | 6mL | 3mL | 無 |
|
|
|
|
檢測抗體-HRP | 10mL | 5mL | 無 |
|
|
|
|
20×洗滌緩沖液 | 25mL | 15mL | 按說明書進(jìn)行稀釋 |
|
|
|
|
底物 A | 6mL | 3mL |
|
| 無 |
|
|
|
|
|
|
底物 B | 6mL | 3mL |
|
| 無 |
|
|
|
|
|
|
終止液 | 6mL | 3mL |
|
| 無 |
|
|
|
|
| |
封板膜 | 2 張 | 2 張 |
| 無 | |
|
|
|
|
| |
說明書 | 1 份 | 1 份 |
| 無 | |
|
|
|
|
| |
自封袋 | 1 個 | 1 個 |
| 無 | |
|
|
|
|
|
|
注:標(biāo)準(zhǔn)品濃度依次為:120、60、30、15、7.5、0 | μmol/L. |
|
試劑的準(zhǔn)備
20×洗滌緩沖液的稀釋:蒸餾水按 1:20 稀釋,即 1 份的 20×洗滌
緩沖液加 19 份的蒸餾水。
洗板方法
操作步驟
L;
OD 值。
結(jié)果判斷
繪制標(biāo)準(zhǔn)曲線:在 Excel 工作表中,以標(biāo)準(zhǔn)品濃度作橫坐標(biāo),對應(yīng)
OD 值作縱坐標(biāo),繪制出標(biāo)準(zhǔn)品線性回歸曲線,按曲線方程計(jì)算各樣 | 5. | 貯藏:2-8℃,避光防潮保存。 |
本濃度值。 | 6. | 有效期:6 個月 |
| 免責(zé)聲明 | |
| 1. | 試劑盒僅供研究使用,不得用于臨床實(shí)驗(yàn)或人體實(shí)驗(yàn),否則所 |
| 產(chǎn)生的一切后果,由實(shí)驗(yàn)者承擔(dān),本公司概不負(fù)責(zé)。 | |
| 2. | 嚴(yán)格按照說明書操作,實(shí)驗(yàn)者違反說明書操作,后果由實(shí)驗(yàn)者 |
| 承擔(dān)。 |
試劑盒性能
0.9900。
FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Rat ONOO- (ONOO-) ELISA Kit instruction
Intended use
This ONOO- ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ONOO- in the sample, this ONOO- ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ONOO- concentration. The concentration of ONOO- in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storages
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.
Materials required but not supplied
Precautions
Remove all kit reagents from refrigerator and allow them to reach room temperature
( 20-25°C)
Materials supplied
Name | 96 determinations | 48 determinations |
|
|
|
Microelisa stripplate | 12*8strips | 12*4strips |
Standard | 0.3ml | 0.3ml |
|
|
|
Sample diluent | 6.0ml | 3.0ml |
HRP-Conjugate reagent | 10.0ml | 5.0ml |
|
|
|
20X Wash solution | 25ml | 15ml |
|
|
|
Chromogen Solution A | 6.0ml | 3.0ml |
|
|
|
Chromogen Solution B | 6.0ml | 3.0ml |
|
|
|
Stop Solution | 6.0ml | 3.0ml |
|
|
|
Closure plate membrane | 2 | 2 |
|
|
|
User manual | 1 | 1 |
|
|
|
Sealed bags | 1 | 1 |
Note: Standard concentration was followed by: 120、60、30、15、7.5、0 μmol/L.
Reagent preparation
20×wash solution:Dilute with Distilled or deionized water 1:20.
Assay procedure
1. Prepare all r e a g e n t s before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.
環(huán)保在線 設(shè)計(jì)制作,未經(jīng)允許翻錄必究 .? ? ?
請輸入賬號
請輸入密碼
請輸驗(yàn)證碼
請輸入你感興趣的產(chǎn)品
請簡單描述您的需求
請選擇省份