當前位置：廈門慧嘉生物科技有限公司>>ELISA試劑盒 >>小鼠的ELISA試劑盒 >>優(yōu)品質小鼠（GR）ELISA試劑盒小鼠糖皮質類固醇受體（GR）ELISA試劑盒

優(yōu)品質小鼠（GR）ELISA試劑盒小鼠糖皮質類固醇受體（GR）ELISA試劑盒

返回列表頁

參考價

面議

具體成交價以合同協(xié)議為準

產品型號

品牌

廠商性質代理商

所在地廈門市

在線詢價收藏產品查看聯(lián)系電話

聯(lián)系方式：陳先生查看聯(lián)系方式

更新時間：2018-05-18 02:47:41瀏覽次數：628次

聯(lián)系我時，請告知來自環(huán)保在線

產品分類 品牌分類

  ELISA試劑盒

ELISA試劑盒綜合大全

人的ELISA試劑盒

小鼠的ELISA試劑盒

大鼠的ELISA試劑盒

雞的ELISA試劑盒

牛的ELISA試劑盒

豚鼠的ELISA試劑盒

猴的ELISA試劑盒

其它的ELISA試劑盒

豬的ELISA試劑盒

兔的ELISA試劑盒

全部產品列表

暫無信息

廈門慧嘉生物科技有限公司

經營模式：代理商

商鋪產品：9325條

所在地區(qū)：福建廈門市

聯(lián)系人：陳先生

詢價 給他留言

產品簡介

廈門慧嘉生物公司*經營小鼠糖皮質類固醇受體(GR)ELISA試劑盒\(zhòng)Santa\Abcam\Cst\jackson \Pierce\Sigma\Amresco\Qiagen\Cayman\abnova\millipore\invitrogen\ merk\ebioscience \prospec\peprotech細胞因子*

詳細介紹

廈門慧嘉生物*專業(yè)銷售ELISA試劑盒\(zhòng)Santa \ Abcam \ Cst \ jackson \ Pierce \ Sigma \ Amresco \Qiagen \ Cayman \ abnova \ millipore \ invitrogen \ merk \ ebioscience \prospec \ peprotech細胞因子*。等生物試劑產品。實驗為大，誠信經營，為客戶提供“zui高質量的產品”和“的服務: : 或登陸http://www.biohj.com（向客服人員索取原版說明書）。歡迎廣大老師來詢！”

小鼠糖皮質類固醇受體（GR）ELISA試劑盒

小鼠黃體生成素釋放激素（LHRH）ELISA試劑盒

小鼠α半乳糖基抗原（α-Gal）ELISA試劑盒

小鼠β氨基己糖苷酶A（β-Hex A）ELISA試劑盒

小鼠肺表面活性物質相關蛋白A（SP-A）ELISA試劑盒

小鼠克拉拉細胞蛋白（CC16）ELISA試劑盒

小鼠β半乳糖苷酶（βGAL）ELISA試劑盒

小鼠腎上腺素（EPI）ELISA試劑盒

小鼠腎素（Renin）ELISA試劑盒

小鼠一氧化氮（NO）ELISA試劑盒

小鼠雌三醇（E3）ELISA試劑盒

檢測范圍：	Request Information
靈敏度：	Request Information
反應時間：	1-3h
所需樣本體積：	50-100ul
檢測波長：	450 nm
說明書：	（向客服人員索取原版說明書）
原理：	This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase （HRP） is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.
特異性：	This assay has high sensitivity and excellent specificity for detection of Rat ACHE. No significant cross-reactivity or interference between Rat ACHE and analogues was observed.
精密度：	Intra-assay Precision （Precision within an assay）: CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision （Precision between assays）: CV%<10% Three samples of known concentration were tested in twenty assays to assess.
樣本搜集及儲存：	Serum: Use a serum separator tube （SST） and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
檢測步驟：	Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody （1x） to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. （Biotin-antibody （1x） may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.） 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer （200μl） using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin （1x） to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
結果計算：	Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ACHE concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

廈門慧嘉生物*專業(yè)銷售ELISA試劑盒\(zhòng)Santa \ Abcam \ Cst \ jackson \ Pierce \ Sigma \ Amresco \Qiagen \ Cayman \ abnova \ millipore \ invitrogen \ merk \ ebioscience \prospec \ peprotech細胞因子*。等生物試劑產品。實驗為大，誠信經營，為客戶提供“zui高質量的產品”和“的服務”: : 或登陸http://www.biohj.com（向客服人員索取原版說明書）。歡迎廣大老師來詢！