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IFN γ Porcine/重組豬干擾素γ

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IFN γ Porcine/ 重組豬干擾素γ 產(chǎn)品編號：CYT-402
產(chǎn)品規(guī)格：10μg/50μg/1mg

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IFN γ Porcine/ 重組豬干擾素γ

產(chǎn)品編號：CYT-402
產(chǎn)品規(guī)格：10μg/50μg/1mg
 

Synonyms Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Introduction IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Description Interferon-gamma Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 17140 Dalton.
The IFN-gamma is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The protein was lyophilized with no additives.
Solubility It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interferon-g although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Cys-Gln-Ala.
Biological Activity The ED50 as determined by the amount of interferon that inhibited 50% of the cytopathic effect of vesicular stomatitis virus in MDBK cells was found to be 0.2-0.6 ng/ml.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.556 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.

2. Analysis by RP-HPLC, using a calibrated solution of IFNg as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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