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IL 18 Rat/重組大鼠白介素18 產(chǎn)品編號：CYT-376

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IL 18 Rat/重組大鼠白介素18 產(chǎn)品編號：CYT-376
產(chǎn)品規(guī)格：5μg/25μg/1mg

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IL 18 Rat/重組大鼠白介素18

產(chǎn)品編號：CYT-376
產(chǎn)品規(guī)格：5μg/25μg/1mg

Synonyms	Interferon-gamma-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin.
Introduction	IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein （IL18BP） can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Description	Interleukin-18 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton. The IL-18 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	Lyophilized from a concentrated （1mg/ml） solution in water containing 0.4 x PBS pH=7.2.
Solubility	It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Amino acid sequence	The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu.
Biological Activity	The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells （4 x 105 cells/well） co-stimulated with sub optimal dose of ConA （0.75ug/ml） is 12 ng/ml. The medium was RPMI containing 10% FCS and 50μM 2-ME.
Protein content	Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.213 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-18 as a Reference Standard.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.