anti-BAX 抗體說明書
Catalog Number : Rs-0127R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t（14;18）-bearing follicular B cell lymphomas（1,2）.Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal （3,5）.Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action （6,7）.A more recently identified protein,designated Bax p21（i.e., Bcl-associated X protein ）,has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2（8）.Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2（8）.
Specificity :
· anti- BAX is a rabbit monoclonal antibody unconjugated
· specific for BAX of human, mo, rat, bovine, sheep, dog, pig
· broadly reactive with mammalian species
· epitope mapping near the amino terminus of BAX of
human, mo, rat, bovine, sheep, dog, pig origin
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 21kDa
Application:
· Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage:
Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
anti-BAX 抗體說明書
