Anti-VWF抗體說(shuō)明書(shū)
Quantity size: 0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: Von Willebrand Factor （VWF） was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a plaet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in plaet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to plaet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding （gingival, minor skin quantitative lacerations, menorrhagia, etc.）.
Anti-VWF抗體說(shuō)明書(shū)
Specificity: · Anti-VWF is a rabbit monoclonal antibody unconjugated
· specific for VWF of rat
· epitope mapping near the C terminus of VWF of rat
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG · mol wt: 48kDa
Application: · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-VWF抗體說(shuō)明書(shū)
