Mouse Anti-EGF
(Epidermal growth factor)to human
Catalog Number : Rs-2008R
Quantity size : 0.2ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
Specificity : · anti- EGF is a Mouse monoclonal antibody, unconjugated
· specific for EGF of human
· epitope mapping in the middle of EGF of human
· Protein G affinity chromatography purification, purity :>96%
· Isotype: IgG
· use for western blotting, elisa, immunoprecipitation and
immunohistochemistry
· mol wt: 161kDa
Mouse Anti-EGF
Application: · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mouse Anti-EGF