Anti-IL-4R/CD124抗體說(shuō)明書

Catalog Number : Rs-1355R

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

Background:

This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. [provided by RefSeq, Jul 2008].Function : Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE

production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Soluble IL4R （sIL4R） inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

pecificity :   

-  Anti-IL-4R  is a mouse  monoclonal  antibody unconjugated                

specific for CD124 of  Human, Rat

• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:201-222kDa

Application :  

• WB=1:100-500 ELISA=1:500-1000 IP=1:20-100  IHC-P=1:100-500 IHC-F=1:100-500  IF=1:100-500
  not yet tested in other applications.
• optimal dilutions/concentrations should be determined by the end user..

Storage:  Store at -2Anti-IL-4R/CD124抗體說(shuō)明書0℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.