Background: The detection of noxious stimuli （chemical, mechanical, or thermal） occurs predominantly at the peripheral terminals of primary afferent neurons. This information is ultimay transmitted to the central nervous system to evoke a perception of pain which initiates appropriate protective reflexes. The receptor for capsaicin, VR4 （vanilloid receptor 1; TRPV1 is a nonselective cation channel that resembles members of the transient receptor potential （TRP） family of ion channels. The vanilloid receptor 1 protein functions both as a receptor for capsaicin and a transducter of noxious thermal stimuli. VR4 protein is localized to small-diameter sensory neurons within the dorsal root ganglia and nerve terminals in the dorsal horn.

· anti-VR4 is a rabbit monoclonal antibody unconjugated

· specific for VR4 of hu, mo, rat, Gpig, dog

· epitope mapping near the amino terminus of VR4 of human, mo, rat origin

· Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 92kDa

· Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.