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Sample collection and storages
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximay 3000×g. Remove serum and assay immediay or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
人巨噬細(xì)胞來源的趨化因子(MDC)ELISA試劑盒Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediay or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequay and no hemolysis or granule was allowed.
人巨噬細(xì)胞來源的趨化因子(MDC)ELISA試劑盒Materials required but not supplied
1. Standard microplate reader(450nm)
2. Precision pipettes and Disposable pipette tips.
3. 37 ℃ incubator
Precautions
1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
3. Mix all reagents before using.
Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)
Reagent preparation
20×wash solution:Dilute with Distilled or deionized water 1:20.
甲砜霉素 2,2-二氯-N-[(1R,2R)1-(羥基甲基)-2-羥基-2-[4-(甲基磺?;?苯基]乙酰胺;甲砜氯霉素;硫霉素; Thiamphenicol 試劑級,98% 25克 15318-45-3 2~8℃
100克
500克
非諾貝特 2-甲基-2-(4-(4-氯苯甲酰基)苯氧基)丙酸異丙酯;苯酰降脂丙酯;立平脂;普魯脂芬;FenofibZIKERBte 試劑級,98% 5克 49562-28-9 RT
25克
100克
喹乙醇 奧拉喹多;快育靈;奎乙醇;奧拉多司;2-(N-2-羥yi基-氨基甲酰)-3-甲基喹喔啉-1,4-二氧化物;N-羥yi基-3-甲基-2-喹啉酰胺-1,4-二氧化物;奧喹多司;Josamycin 試劑級,98% 1克 23696-28-8 RT
5克
復(fù)達(dá)欣 頭孢他啶;頭孢他定;頭孢噻甲羧肟;頭孢塔齊定;噻甲酸肟頭孢菌素;凱復(fù)定;(6R,7R)-7-(((2-氨基-4-噻唑基)-((1-羧基-1-甲基乙氧基)亞氨基)乙酰基)氨基)-2-羧基-8-氧代-5-硫雜-1-氮雜雙環(huán)(4.2.0)辛-2-烯-3-甲基吡啶鎓內(nèi)鹽;頭孢齊定;Ceftazidime 試劑級,88% 1克 72558-82-8 2~8℃
5克
25克
去氧野艽霉素鹽酸鹽 脫氧野尻霉素鹽酸鹽;脫氧野尻霉素;鹽酸脫氧野尻霉素;1-脫氧野尻霉素;(2R,3R,4R,5S)-2-羥甲基哌啶-3,4,5-三醇;1-DNJ;DNJ 試劑級,98% 10毫克 73285-50-4 2~8℃
鹽酸氨丙林 鹽酸氨丙啉;1-(4-氨基-2-丙基-5-嘧啶甲基)-2-甲基吡啶氯化物鹽酸鹽;鹽酸安普羅胺;鹽酸安普霉素;鹽酸安普羅銨;鹽酸安普羅林;氨丙基嘧吡啶;氨丙嘧吡啶;Amprolium hydrochloride 試劑級,99% 25克 137-88-2 RT
100克
酒石酸泰樂菌素 泰樂二甲基酸;酒石酸太東菌素;酒石酸泰洛星;Tylosin tartZIKERBte 試劑級,800U/mg 1克 74610-55-2 2~8℃
5克
25克
泰樂霉素 酒石酸泰樂菌素;泰樂二甲基酸;酒石酸太東菌素;酒石酸泰洛星;Tylosin tartZIKERBte 試劑級 250毫克 1405-54-5 2~8℃