［產(chǎn)品名稱］大鼠胰島素（Insulin）ELISA檢測試劑盒
［英文名稱］Rat Insulin ELISA KIT
［貨號］ZK-R3059
［試劑盒］保存條件、保質(zhì)期2-8℃低溫保存下，6個月的保質(zhì)期。
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大鼠胰島素（Insulin）ELISA檢測試劑盒購買子科生物ELISA系列產(chǎn)品優(yōu)勢：
1）每個產(chǎn)品出庫前都會進行質(zhì)檢，質(zhì)檢合格后才可出庫，以確保每個產(chǎn)品質(zhì)量合格，以避免出現(xiàn)售后服務(wù)。
2）產(chǎn)品經(jīng)過技術(shù)人員多年的研發(fā)經(jīng)驗調(diào)試和修正，讓產(chǎn)品實驗效果穩(wěn)定，靈敏度高，重復性高，吸附性能好，空白值低，孔底透明度高，且*。
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4）快速，檢測時間短（2-3小時即可完成），幫您節(jié)省寶貴的實驗時間。
5）免費提供ELISA試劑盒免費代測服務(wù)，和數(shù)據(jù)分析工作。需要代測的客戶只需要準備好您的樣本，郵寄到我們公司實驗室即可，本市可以安排工作人員上門取樣本，我們承諾在5個工作日內(nèi)給您zui終檢測結(jié)果。
6）適用范圍廣：可以檢測血清、血漿、各組織樣本、細胞培養(yǎng)上清液、尿液、腦脊液、糞便等等多種標本類型；
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Assay procedure
1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 
5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not 
appear uniform, gently tap the plate to ensure thorough mixing.
8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.

Calculation of results
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 
2.First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.The sensitivity by this assay is 10.0 pg/ml
6.Standard curve
Storage：  2-8℃.
validity： six months.

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