中文名稱：羧基-PEG-二苯基環(huán)辛炔,羧基聚乙二醇二苯基環(huán)辛炔
英文名稱：DBCO-PEG-COOH，DBCO-PEG-ACID,Dibenzocyclooctyne-PEG-Acid
別名：DBCO-PEG-COOH
分子量：400、600、1K、2K、3.4K、4K、5K、10K
純度：95%以上
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DBCO-PEG-COOH, is a heterobifunctional reactive PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneously without the need for toxic Cu catalysts. Carboxylic acid group, on the other hand, can be activated to react with amines, hydroxy and other functional groups.
Copper-free Click reaction procedures:
Prepare the azide-containing reagents in reaction buffer.
Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.
Purify conjugates either by dialysis or size-exclusion chromatograph.
