上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-P4HB抗體，我公司銷售國內(nèi)外科研產(chǎn)品，如生物試劑、生化試劑，一抗二抗，各種標(biāo)記抗體，Elisa酶聯(lián)免疫試劑盒，放免試劑盒，培養(yǎng)基，各種動物血清等產(chǎn)品，歡迎廣大新老客戶前來咨詢訂購，訂購：或。

蛋白質(zhì)二硫鍵異構(gòu)酶抗體,Anti-P4HB抗體簡單介紹：

【中文名稱】：蛋白質(zhì)二硫鍵異構(gòu)酶抗體

【英文名稱】：Anti-P4HB抗體

【英文別名】：Cellular thyroid hormone binding protein; Cellular thyroid hormone-binding protein; Collagen prolyl 4 hydroxylase beta; Disulphide Isomerase; DSI; EC 5.3.4.1; Endoplasmic reticulum resident protein 59; ER protein 59; ERBA2L; ERp59; GIT; Gltathione insulin transhydrogenase; Glutathione insulin transhydrogenase; P4Hbeta; p55; PDI; PDIA1; PDIA1_HUMAN; PDIR; PHDB; PO4DB; PO4HB; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1); Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit; PROHB; Prolyl 4 hydroxylase beta polypeptide; Prolyl 4 hydroxylase beta subunit; Prolyl 4 hydroxylase subunit beta; Prolyl 4-hydroxylase subunit beta; Protein disulfide isomerase associated 1; Protein disulfide isomerase, family A, member 1; Protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; Protocollagen hydroxylase; Thbp; Thyroid hormone binding protein p55; Thyroid hormone binding protein p55 cellular; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like.  

【規(guī)  格】：0.1ml/0.2ml

【相關(guān)標(biāo)記】：Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.【濃    度】：1mg/1ml

【抗體來源】：Rabbit or Mouse or Goat 

【克隆類型】：polyclonal or monoclonal

【產(chǎn)品類型】：一抗

【產(chǎn)品應(yīng)用】：可以用于做石蠟切片免疫組化，冰凍切片免疫組化，Elisa，WB，免疫熒光、流式細(xì)胞術(shù)等實驗。

蛋白質(zhì)二硫鍵異構(gòu)酶抗體【背景介紹】：The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

蛋白質(zhì)二硫鍵異構(gòu)酶抗體,Anti-P4HB抗體免疫組化操作流程：

（一） 儀器設(shè)備
1. 18cm不銹鋼高壓鍋或電爐或用微波爐.
2. 水浴鍋
（二） 試 劑
1. PBS緩沖液（ph7.2―7.4）：NaC137mmol/L，KCl 2.7mmol/L ,Na2HPO4 4.3mmol/L,
KH2PO4 1.4mmol/L.
2．0.01mol/L檸檬酸鈉緩沖液（CB,ph6.0,1000ml）：檸檬酸三鈉3g,檸檬酸0.4g。
3．0.5mol/L EDTA緩沖液（ph8.0）：700ml水中溶解186.1g EDTA&
8226;2H2O,用10mmol/L
NaOH調(diào)至ph8.0,加水至1000ml.
4. 1mol/L的TBS緩沖液（ph8.0）：在800ml水中溶解121gTris堿，用1N的HCl調(diào)至ph8.0,加水1000ml。
5. 酶消化液：a. 0.1%*：用0.1%CaCl 12（ph7.8）配制。b.0.4%*液：用0.1N的HCl配制。
6. 3%甲醇―H2O2溶液：用30%H2O2和80%甲醇溶液配制
7. 風(fēng)裱劑：a. 甘油和0.5mmol/L碳酸鹽緩沖液（ph9.0–9.5）等量混合
b 油和TBS（PBS）配制
8．TBS/PBS PH9.0–9.5,適用于熒光纖維鏡標(biāo)本;ph7.0-7.4適合光學(xué)纖維標(biāo)本。
（三） 操作流程
1 脫蠟和水化：脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復(fù)：用于福爾馬林固定的石蠟包埋組織芯片

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相關(guān)標(biāo)記抗體：HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體

相關(guān)優(yōu)質(zhì)產(chǎn)品：

Cyclin A  周期素A抗體
Cyclin B1  周期素B1抗體
Cyclin C  周期素C抗體
Cyclin D1  周期素D1抗體
Cyclin D2  周期素D2抗體
Cyclin D3(human, mo, rat, pig, cattle, dog, Rb)  周期素D3抗體
Cyclin E  周期素E/原癌基因抗體
Cyclin F  周期素F抗體
Cyclin G  周期素G抗體
Cyclin H  周期素H抗體
Cygb (cytoglobin)  細(xì)胞球蛋白抗體
CYP1A1(Cytochrome p450 1A1)  細(xì)胞色素P450 1A1抗體
P450 1A2(Cytochrome P450 1A2)  細(xì)胞色素P450 1A2抗體
CYP3A4(Cytochrome p450 3A4)  抗細(xì)胞色素P450 3A4抗體
CYP19(Cytochrome p19)  細(xì)胞色素P450 19抗體
CYP21(Cytochrome P450 21; cytochrome P450, family 21, subfamily A, polypeptide 2)  21-羥化酶抗體
CYP450(Cytochrome P450 monooxygenase)  細(xì)胞色素P450單氧化酶抗體
Cyr61/IGFBP10/CCN1(cysteine rich 61)  富半*肝素結(jié)合蛋白61抗體
CYP2E1(cytochrome P450 2E1)  抗細(xì)胞色素P450ⅡE1抗體
Cytochrome C  細(xì)胞色素 C抗體
DRD1 receptor (dopamine D1 receptor)  多巴胺受體-D1抗體
DRD2 receptor(dopamine D2 receptor)  多巴胺受體-D2抗體
DRD3 receptor(dopamine D3 receptor)  多巴胺受體-D3抗體