上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-C1orf76抗體,我公司銷售國(guó)內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購(gòu),訂購(gòu): 。
神經(jīng)母細(xì)胞瘤源性分泌蛋白抗體,Anti-C1orf76抗體簡(jiǎn)單介紹
【中文名稱】:神經(jīng)母細(xì)胞瘤源性分泌蛋白抗體
【英文名稱】:Anti-C1orf76抗體
【別 名】:C1orf76; NDSP; FAM163A; Chromosome 1 open reading frame 76; F163A_HUMAN; Fam163a; hypothetical protein MGC16664; NDSP; Neuroblastoma derived secretory protein; Neuroblastoma-derived secretory protein; Protein FAM163A
【規(guī) 格】:0.1ml/0.2ml
【相關(guān)標(biāo)記】:Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.
【濃 度】:1mg/1ml
【抗體來源】:Rabbit or Mouse or Goat
【克隆類型】:polyclonal or monoclonal
神經(jīng)母細(xì)胞瘤源性分泌蛋白抗體背景介紹:Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear and is a topic of continuing exploration. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1. A breakpoint has been identified in 1q which disrupts the DISC1 gene and is linked to schizophrenia. Aberrations in chromosome 1 are found in a variety of cancers including head and neck cancer, malignant melanoma and multiple myeloma. The C1orf76 gene product has been provisionally designated C1orf76 pending further characterization.
【產(chǎn)品類型】:一抗
【產(chǎn)品應(yīng)用】:可以用于做石蠟切片免疫組化,冰凍切片免疫組化,Elisa,WB,免疫熒光、流式細(xì)胞術(shù)等實(shí)驗(yàn)。
抗體修復(fù)方式:
修復(fù)液:0.01M 枸櫞酸(pH6.0)或者0.05M EDTA(pH8.0)
抗體修復(fù)方法:
方法1:沸水浴修復(fù),將盛有修復(fù)液和玻片的燒杯置于沸水浴環(huán)境,保持外部沸騰狀態(tài)15min,自然冷卻至室溫;
方法2:微波修復(fù),將盛有修復(fù)液和玻片的燒杯置于微波爐中,高火5min,?;?min,中火5min,自然冷卻至室溫。
神經(jīng)母細(xì)胞瘤源性分泌蛋白抗體,Anti-C1orf76抗體免疫組化操作步驟:
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風(fēng)裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標(biāo)本;pH7.0-7.4適合光學(xué)纖維標(biāo)本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
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LIS1(Lissencephaly-1 protein) 無腦回的致病基因LIS1抗體
LIX1 LIX1抗體
Livin (InhibitoBYK of apoptosis proterins Livin) 一種新的凋亡抑制蛋白抗體
LKB1 一種抑癌基因(*/*蛋白激酶)
LFABP/FABP-1(Liver Fatty acid binding protein) 肝臟型脂肪酸結(jié)合蛋白抗體
LFABP/FABP-2(Liver Fatty acid binding protein) 肝臟型脂肪酸結(jié)合蛋白抗體
LN (laminin) 層粘連蛋白抗體
Laminin β1 層粘連蛋白β1抗體
lamin A 核纖層蛋白A抗體
Lamin B 核纖層蛋白B抗體
lamin C 核纖層蛋白C抗體
LOX-1/OLR1(Lectin-type oxidized LDL receptor 1) 凝集素樣氧化型低密度脂蛋白受體抗體
Lpin1 protein Lpin1 抗體
Lpin1 protein Lpin1 抗體
LPL(lipoprotein lipase) 脂蛋白脂酶抗體
LPLUNC1(long palate, lung and nasal epithelium carcinoma associated 1 isoform 1) 鼻咽癌癌基因抗體
LRIG1(leucine rich repeats and immunoglobulin-like domains 1) 抗LRIG1抗體
LRIG2(leucine rich repeats and immunoglobulin-like domains 2) 抗LRIG2抗體
LRIG3(leucine rich repeats and immunoglobulin-like domains 3) 抗LRIG3抗體