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相關標記抗體：HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體

上海研盟生物科技有限公司Anti-Unc5a抗體*，主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取?？头?/span>

英文名稱：Anti-Unc5a antibody

中文名稱：神經(jīng)突起生長誘導因子受體Unc5a抗體

神經(jīng)突起生長誘導因子受體Unc5a抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

★研盟生物★神經(jīng)突起生長誘導因子受體Unc5a抗體,Anti-Unc5a抗體

免疫組化操作步驟

（一）、儀器設備

1、 18cm不銹鋼高壓鍋或電爐或用微波爐

2、 水浴鍋

（二）、試 劑

1、 PBS緩沖液（pH7.2―7.4）

2、 0.01mol/L檸檬酸鈉緩沖液（CB,pH6.0,1000ml）

3、 3%甲醇―H2O2溶液：用30% H2O2 和80%甲醇溶液配制

4、 風裱劑：

a. 甘油和0.5mmol/L碳酸鹽緩沖液（pH9.0–9.5）等量混合

b. 油和TBS（PBS）配制

5、 TBS/PBS pH9.0–9.5，適用于熒光纖維鏡標本；pH7.0-7.4適合光學纖維標本

（三）、操作流程

1、 脫蠟和水化：脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘

a. 組織芯片置于二甲苯中浸泡10分鐘，更換二甲苯后在浸泡10分鐘

b. 無水乙醇中浸泡五分鐘

c. 95%乙醇中浸泡五分鐘

d. 75%乙醇中浸泡五分鐘

2、 抗原修復：用于福爾馬林固定的石蠟包埋組織芯片

神經(jīng)突起生長誘導因子受體Unc5a抗體,Anti-Unc5a抗體溶解方法:

方法1：我們的抗體（凍干粉）已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑（疊氮鈉或*），您只需要加入滅菌的雙蒸水就行了?？贵w溶解后，置于-20℃保存，分裝后使用，避免反復凍融，可保證至少1年有效；

方法2：也可以只加入一半體積的雙蒸水，再用一半體積的甘油補足，置于-20℃保存，這樣抗體不會凍，有利于抗體的穩(wěn)定。

后續(xù)試驗時，再使用對應的緩沖液稀釋成工作液，即用即配。

我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（*），至少三年有效。

Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:

1) Reconstituting by directly adding 100uL of sterile, distilled water.

2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.

簡單介紹：

產(chǎn)品別名：UNC5H1; Netrin receptor UNC 5A; Netrin receptor UNC5A; Netrin receptor Unc5h1; Protein unc-5 homolog 1; Protein unc-5 homolog A; Unc 5 homolog 1; Unc 5 homolog A; Unc 5a; Unc5 a; Unc5 homolog 1; Unc5 homolog A; Unc5a; UNC5A_HUMAN; Unc5h1; FLJ16449; KIAA1976.      

抗體來源：Rabbit or Mouse

保質(zhì)期:1年

克隆類型：Polyclonal or monoclonal

性    狀：Lyophilized or Liquid

濃    度：1mg/1ml

背景介紹：The UNC5H family of proteins act as transmembrane receptors for netrin-1 and play a crucial role in axon guidance and migration of neural cells. Additionally, when cleaved by a caspase to produce an intracellular fragment containing a death domain,UNC5H receptors induce apoptosis. This activity is blocked by the binding of netrin-1. In the absence of netrin-1, UNC5H receptors act as tumor suppressors by inhibiting anchorage-independent growth and invasion, but mutation of these receptors provides a potential mechanism for tumorigenicity. The expression of UNC5H receptors is downregulated in multiple carcinomas, including colorectal, breast, ovary, uterus, stomach, lung and kidney cancers. UNC5H1, also known as UNC5HA (unc-5 homolog A), is a member of the UNC5H family of proteins and is localized to the cell membrane. Three isoforms of UNC5H1 exist due to alternative splicing events.

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