上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-DFFB抗體，我公司銷售國(guó)內(nèi)外科研產(chǎn)品，如生物試劑、生化試劑，一抗二抗，各種標(biāo)記抗體，Elisa酶聯(lián)免疫試劑盒，放免試劑盒，培養(yǎng)基，各種動(dòng)物血清等產(chǎn)品，歡迎廣大新老客戶前來咨詢訂購(gòu)，訂購(gòu):或。

DNA斷裂因子抗體,Anti-DFFB抗體簡(jiǎn)單介紹：

中文名稱：DNA斷裂因子抗體（蛋白酶激活脫氧核糖核酸酶）

英文名稱：Anti-DFFB抗體

別 名：CAD; Caldesmon; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; CDM; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF2; DFF40; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNAation factor, beta subunit; DFFB_HUMAN.    

此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記

背景介紹：Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. 

規(guī)格：0.1ml/0.2ml

濃度：1mg/1ml

抗體來源：本公司抗體有兔來源和鼠來源等

克隆類型：兔來源為多抗，鼠來源為單抗

產(chǎn)品類型：一抗

DNA斷裂因子抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

避免反復(fù)凍融，保質(zhì)期一年。

DNA斷裂因子抗體,Anti-DFFB抗體免疫組化問題解答

1、染色過強(qiáng)？

原 因：抗體濃度過高或孵育時(shí)間過長(zhǎng)

解決方法：降低抗體滴度、抗體孵育時(shí)間：室溫1小時(shí)或4度過夜

原 因：孵育溫度過高超過37度

解決方法：一般在室溫20-28度

原 因：DAB顯色時(shí)間過長(zhǎng)或濃度過高

解決方法：顯色時(shí)間不超過5-12分鐘，以顯微鏡下觀察為準(zhǔn)

2、非特異性背景染色？

原 因：操作過程中沖洗不充分

解決方法：每步?jīng)_洗3次每次5分鐘

原 因：組織中含過氧化物酶未阻斷

解決方法：可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)

原 因：組織中含內(nèi)原性*

解決方法：正常非免疫動(dòng)物血清再封閉

原 因：血清蛋白封閉不充分

解決方法：血清蛋白封閉不充分

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