產(chǎn)品名稱： 多黏類芽孢桿菌價(jià)格
英文名稱：Paenibacillus polymyxa
型號(hào)：CS-JZ0001
培養(yǎng)保藏法:培養(yǎng)后于4—6℃冰箱內(nèi)保存。
庫(kù)存：提供現(xiàn)貨 
保存：低溫避光保存
多黏類芽孢桿菌價(jià)格脫水保存法：
①沙土保存法，能產(chǎn)孢子的細(xì)菌、放線菌及霉菌可采用此法保存,即將沙和土以3:2比例混合，經(jīng)稀酸處理洗凈過(guò)篩,裝入小試管內(nèi),裝置高度為1cm;滅菌2～3次，烘干后即可將在斜面培養(yǎng)基上生長(zhǎng)良好的孢子，用無(wú)菌蒸餾水2～2.5ml制成孢子懸液，吸取少許加入沙土管中，經(jīng)真空抽干，外觀呈松散狀態(tài),于4℃冰箱保存，保存期可達(dá)5～7年。
② 冷凍干燥法,將孢子懸浮液與保護(hù)劑（一般用脫脂牛奶或血清）相混合，放在安瓿管內(nèi)于-20～-30℃的乙醇浴中速凍。然后，在低溫下用真空泵抽干，并用五氧化二磷或干冰使水汽結(jié)凍，熔封安瓿管，于低溫下保存。保存期可達(dá)5～10年之久。
③ 石蠟油封存法，在斜面菌種培養(yǎng)物上,倒上滅菌后的石蠟油，高出斜面1cm，于4℃冰箱或低溫干燥處保存，此法不適用于能利用石蠟油作碳源的微生物，保存期為一年以上。

低溫保存法： 
1.簡(jiǎn)單保存法，將瓊脂斜面孢子培養(yǎng)物、菌絲懸浮液以及由麩皮、大米、小米等谷物原料制成的孢子培養(yǎng)物置于4℃冰箱保存,保存時(shí)間不超過(guò)1～2個(gè)月，若將谷物原料制備的孢子瓶抽真空并在棉塞上浸蠟,以隔絕外界空氣和水汽,保持時(shí)間可達(dá)3～4個(gè)月。 
2.液氮超低溫保存法，將生長(zhǎng)穩(wěn)定期的細(xì)胞懸浮在10%甘油或其他低冰點(diǎn)液體中，密封于安瓿管內(nèi)，然后控制冷卻速度，使安瓿管溫度逐步下降至-35℃時(shí)，即可置于-150～-196℃的液氮罐中保存。大多數(shù)微生物如病毒、噬菌體、多種細(xì)菌、放線菌、酵母和原蟲、特別是一些用冷凍干燥法有困難的微生物，都可用此法*保存。
操作流程：
菌株復(fù)蘇：（按三區(qū)劃線法將留菌管內(nèi)的原始菌株進(jìn)行復(fù)蘇）
1) 所有菌株，顯色培養(yǎng)基分4區(qū)，每區(qū)一株，標(biāo)明菌株號(hào)、菌種名常用縮寫如下：
cal: 白念珠菌 cgl：光滑念珠菌 cpa: 近平滑念珠菌 ctr: 熱帶念珠菌 ckr: 克柔念珠菌 cne: 新型隱球菌，其他菌種標(biāo)記全名。
2) 隱球菌除傳顯色培養(yǎng)基以外，另傳1/2塊血平皿上述菌株統(tǒng)一37oC孵育48h
菌種鑒定：（48h后觀察結(jié)果）。英文名稱  Anti-GNG13
中文名稱  G蛋白γ13/Gγ13 抗體
別    名  G gamma subunit, clone:h2 35; G(gamma)13; GBG13_HUMAN; Gng13; Guanine nucleotide binding protein 13; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-13; h2 35.
濃    度  1mg/1ml
規(guī) 格  0.2ml/200μg    
抗體來(lái)源  Rabbit 
克隆類型  polyclonal
交叉反應(yīng)  Human, Mouse, Rat, Pig, Cow
產(chǎn)品類型  一抗   
研究領(lǐng)域  細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號(hào) 
蛋白分子量  predicted molecular weight: 8kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human GNG13
亞    型  IgG
純化方法  affinity purified by Protein A
儲(chǔ) 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e. a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alhfa, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the beta gamma delta subunits. It is becoming increasingly clear that different G protein complexes expressed in different tissues carry structurally distinct members of the gamma as well as the alhfa and beta subunits, and that preferential associations between members of subunit families increase G protein functional diversity.
Function : Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Subunit : G proteins are composed of 3 units, alpha, beta and gamma.
Subcellular Location : Cell membrane.
Similarity : Belongs to the G protein gamma family.
Database links : UniProtKB/Swiss-Prot: Q9P2W3.1

英文名稱  Anti-GNG5
中文名稱  G蛋白γ5/Gγ5 抗體
別    名  FLJ92393; GBG5_HUMAN; Gng5; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-5; OTTHUMP00000011474; OTTHUMP00000011565.
濃    度  1mg/1ml
規(guī) 格  0.2ml/200μg    
抗體來(lái)源  Rabbit 
克隆類型  polyclonal
交叉反應(yīng)  Human, Mouse, Rat, Chicken, Cow, Rabbit
產(chǎn)品類型  一抗   
研究領(lǐng)域  細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號(hào) 
蛋白分子量  predicted molecular weight: 7kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human GNG5
亞    型  IgG
純化方法  affinity purified by Protein A
儲(chǔ) 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用   ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.