詳細(xì)介紹
合成寡核苷酸和DNA片段被應(yīng)用于迅速發(fā)展的應(yīng)用領(lǐng)域,包括作為主體或雜交探針用于治療性制劑。沃特世寡核苷酸分離技術(shù)(OST:Oligonucleotide SeparationTechnology)基于BEH雜化顆粒的反相色譜柱,以及Gen-Pak™離子交換柱,應(yīng)對各種高分辨分析與實(shí)驗(yàn)室規(guī)模分離挑戰(zhàn)所需,包括涉及各種DNA和RNA品種。
寡核苷酸分析柱 — 異乎尋常的高分辨率
沃特世寡核苷酸色譜柱裝有鍵合了C18的第二代雜化技術(shù)BEH顆粒。對去三苯甲基(detritylated,或稱脫保護(hù))的合成寡核苷酸樣品的分離,基于成熟的離子對反相色譜法。沃特世提供1.7 µmUPLC顆?;?.5 µm HPLC顆粒,裝填以各種不同色譜柱規(guī)格,從而靈活滿足各種實(shí)驗(yàn)室規(guī)模分離或分析的不同需求,并能實(shí)現(xiàn)異乎尋常的樣品分辨率和的色譜柱使用壽命。此外,沃特世的制造和質(zhì)控測試程序,有助于確保批次之間與柱之間的性能的一致性,而無論應(yīng)用的難度有多高。
■分離效率相當(dāng)于或優(yōu)于PAGE、CGE、或離子交換HPLC方法
■可從去三苯甲基(脫保護(hù))的全長產(chǎn)物中分辨出失敗序列
■可放大的柱規(guī)格,滿足實(shí)驗(yàn)室規(guī)模的分離需求
■超長的色譜柱使用壽命,降低單次分析或分離成本
■經(jīng)MassPREP OST標(biāo)準(zhǔn)品質(zhì)控測試,幫助確保性能穩(wěn)定
Waters oligonucleotide analysis solutions start with our award-winning ACQUITY UPLC systems and industry-leading oligonucleotide columns, which together deliver the ultimate in high-resolution chromatographic performance. Add to this our many detection options, including tunable UV, photodiode array, and fluorescence detectors, as well as nominal mass and high-resolution mass spectrometry equipment—all optimized for UPLC®—and you have a comprehensive set of tools to meet all of your oligonucleotide analysis needs.
This application notebook includes 24 application notes, illustrating work using Waters fit-for-purpose analytical solutions for oligonucleotides, and is organized by:
UPLC-MS for oligonucleotides
UPLC-HRMS for oligonucleotides
UPLC-HDMS for oligonucleotides