LysoBrite 溶酶體近紅外熒光探針，用于標記活細胞的溶酶體。專有的溶解性染料可能通過溶酶體pH梯度選擇性地積聚在溶酶體中。溶質(zhì)指示劑是疏水性化合物，易于滲透完整的活細胞，并在進入細胞后被捕獲在溶酶體中。進入溶酶體后，LysoBrite 試劑的熒光明顯增強。可以使用熒光成像，高含量成像，酶標儀熒光測定法或流式細胞術檢測熒光。 LysoBrite 橙色，紅色，深紅色和NIR試劑具有很高的光穩(wěn)定性和優(yōu)異的細胞保留性，可用于各種研究，包括細胞粘附，趨化性，多藥耐藥性，細胞活力，細胞凋亡和細胞毒性。它們適用于增殖和非增殖細胞，可用于懸浮細胞和貼壁細胞。溶酶體是細胞器，其含有酸水解酶以分解廢物和細胞碎片。溶酶體消化多余的或破舊的細胞器，食物顆粒和吞噬的病毒或細菌。溶酶體周圍的膜允許消化酶在pH4.5下工作。與微堿性胞質(zhì)溶膠（pH 7.2）相比，溶酶體的內(nèi)部是酸性的（pH 4.5-4.8）。溶酶體通過質(zhì)子泵和氯離子通道從細胞質(zhì)中泵送質(zhì)子穿過膜來維持這種pH差異。

2.百螢 LysoBrite溶酶體近紅外熒光探針參數(shù)

Ex(nm) 636

Em(nm) 651

分子量 730.89

溶劑 DMSO

存儲條件在-15℃以下保存，避光防潮

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