MIA-PaCa-2細(xì)胞

細(xì)胞名稱： 人胰腺癌細(xì)胞（STR鑒定正確）

細(xì)胞別名： MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; 

MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2

貨號：TCH-C259

規(guī)格： 1X10^6

參考目錄價格：1600元  (干冰運(yùn)輸需另加200元干冰費(fèi)) 

種屬來源：人

組織來源：胰腺

特征：胰腺癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長特性：貼壁生長

培養(yǎng)體系： DMEM-H+10%FBS+2.5%馬血清+1% Sodium Pyruvate+1%P/S

MIA-PaCa-2細(xì)胞培養(yǎng)基貨號：TCH-G259

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

該細(xì)胞表達(dá)人集落刺激因子CFS-I和血漿酶原激活劑。

MIA-PaCa-2細(xì)胞規(guī)格

參考文獻(xiàn)：

Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455

Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918