好看日韩在线视频免费,日本不卡一区二区三区,三级a全过程在线观看,亚洲精品国产9999久久久久

雅吉生物科技有限公司

人Elisa試劑盒,鼠Elisa試劑盒,豬Elisa試劑盒,細(xì)胞

環(huán)保在線收藏該商鋪

     小標(biāo) 您所在位置:首頁 > 技術(shù)文章 > 人成纖維細(xì)胞生長因子23(FGF-23)Elisa試劑盒說明書
產(chǎn)品搜索

請輸入產(chǎn)品關(guān)鍵字:

聯(lián)系方式
地址:浙江省杭州市西湖區(qū)文二路319號西湖國際科技大廈5號樓中區(qū)3樓
郵編:310012
聯(lián)系人:采購部
留言:在線留言
商鋪:http://www.niunang.cn/st64839/
技術(shù)文章

人成纖維細(xì)胞生長因子23(FGF-23)Elisa試劑盒說明書

點(diǎn)擊次數(shù):181 發(fā)布時(shí)間:2011-11-9

人成纖維細(xì)胞生長因子23(FGF-23)Elisa試劑盒說明書
本試劑盒僅供研究使用。
檢測范圍: 96T
30pg/ml-800pg/ml
使用目的:
本試劑盒用于測定人血清、血漿及相關(guān)液體樣本中FGF-23 含量。
實(shí)驗(yàn)原理
人成纖維細(xì)胞生長因子23(FGF-23)Elisa試劑盒說明書
本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中人FGF-23 水平。用純化的人FGF-23 抗體包被
微孔板,制成固相抗體,往包被單抗的微孔中依次加入FGF-23,再與HRP 標(biāo)記的FGF-23
抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物TMB 顯色。TMB 在
HRP 酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的
FGF-23 呈正相關(guān)。用酶標(biāo)儀在450nm 波長下測定吸光度(OD 值),通過標(biāo)準(zhǔn)曲線計(jì)算樣品
中人FGF-23 濃度。
試劑盒組成
1 30 倍濃縮洗滌液 20ml×1 瓶 7 終止液 6ml×1 瓶
2 酶標(biāo)試劑 6ml×1 瓶 8 標(biāo)準(zhǔn)品(1600pg/ml) 0.5ml×1 瓶
3 酶標(biāo)包被板 12 孔×8 條 9 標(biāo)準(zhǔn)品稀釋液 1.5ml×1 瓶
4 樣品稀釋液 6ml×1 瓶 10 說明書 1 份
5 顯色劑A 液 6ml×1 瓶 11 封板膜 2 張
6 顯色劑B 液 6ml×1/瓶 12 密封袋 1 個(gè)
標(biāo)本要求
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能
馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3 的樣品,因NaN3 抑制辣根過氧化物酶的(HRP)活性。
操作步驟
1. 標(biāo)準(zhǔn)品的稀釋:本試劑盒提供原倍標(biāo)準(zhǔn)品一支,用戶可按照下列圖表在小試管中進(jìn)行稀
釋。
800pg/ml 5 號標(biāo)準(zhǔn)品 150μl 的原倍標(biāo)準(zhǔn)品加入150μl 標(biāo)準(zhǔn)品稀釋液
400pg/ml 4 號標(biāo)準(zhǔn)品 150μl 的5 號標(biāo)準(zhǔn)品加入150μl 標(biāo)準(zhǔn)品稀釋液
200pg/ml 3 號標(biāo)準(zhǔn)品 150μl 的4 號標(biāo)準(zhǔn)品加入150μl 標(biāo)準(zhǔn)品稀釋液
100pg/ml 2 號標(biāo)準(zhǔn)品 150μl 的3 號標(biāo)準(zhǔn)品加入150μl 標(biāo)準(zhǔn)品稀釋液
50pg/ml 1 號標(biāo)準(zhǔn)品 150μl 的2 號標(biāo)準(zhǔn)品加入150μl 標(biāo)準(zhǔn)品稀釋液
2. 加樣:分別設(shè)空白孔(空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、標(biāo)準(zhǔn)孔、
待測樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl,待測樣品孔中先加樣品稀釋液40μl,
然后再加待測樣品10μl(樣品zui終稀釋度為5 倍)。加樣將樣品加于酶標(biāo)板孔底部,盡
量不觸及孔壁,輕輕晃動(dòng)混勻。
3. 溫育:用封板膜封板后置37℃溫育30 分鐘。
4. 配液:將30 倍濃縮洗滌液用蒸餾水30 倍稀釋后備用
5. 洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30 秒后棄去,如此
重復(fù)5 次,拍干。
6. 加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。
7. 溫育:操作同3。
8. 洗滌:操作同5。
9. 顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色
15 分鐘.
10. 終止:每孔加終止液50μl,終止反應(yīng)(此時(shí)藍(lán)色立轉(zhuǎn)黃色)。
11. 測定:以空白空調(diào)零,450nm 波長依序測量各孔的吸光度(OD 值)。 測定應(yīng)在加終止
液后15 分鐘以內(nèi)進(jìn)行。
操作程序總結(jié):
計(jì)算
以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD 值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的
OD 值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD 值計(jì)算出標(biāo)
準(zhǔn)曲線的直線回歸方程式,將樣品的OD 值代入方程式,計(jì)算出樣品濃度,再乘以稀釋倍數(shù),
即為樣品的實(shí)際濃度。
注意事項(xiàng)
1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30 分鐘后方可使用,酶標(biāo)包被板開封后如未
用完,板條應(yīng)裝入密封袋中保存。
2.濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間
控制在5 分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD 值
大于標(biāo)準(zhǔn)品孔*孔的OD 值),請先用樣品稀釋液稀釋一定倍數(shù)(n 倍)后再測定,計(jì)
算時(shí)請zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6.底物請避光保存。
7.嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9.本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準(zhǔn)。
保存條件及有效期
1.試劑盒保存:;2-8℃。
2.有效期:6 個(gè)月
Human fibroblast growth factor-23(FGF-23)
FOR RESEARCH USE ONLY
Assay range:30pg/ml – 800 pg/ml 48 determinations
Purpose
This kit allows for the determination of FGF-23 concentrations in Human serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Human FGF-23 level in the sample,use Purified Human FGF-23 antibody
to coat microtiter plate wells, make solid-phase antibody, then add FGF-23 to wells, Combined
FGF-23 antibody which With HRP labeled goat anti-Human become antibody - antigen -
enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB
substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition
of a sulphuric acid solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of Human FGF-23 in the samples is then determined
by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
1 wash solution 20ml×1bottle 7 Stopp Solution 3ml×1 bottle
2 HRP-Conjugate reagent 3ml×1 bottle 8
Standard
(1600pg/ml)
0.5ml×1 bottle
3 Microelisa stripplate 12well×4strips 9 Standard diluent 1.5ml×1bottle
4 Sample diluent 3ml×1 bottle 10 Instruction 1
5 Chromogen Solution A 3ml×1 bottle 11
Closure plate
membrane
2
6 Chromogen Solution B 3ml×1 bottle 12 Sealed bags 1
RD
Specimen requirements
1. extract as soon as possible after Specimen collection,and according to the relevant
literature, and should be experiment as soon as possible after the extraction. If it can’t,
specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1. Dilute and add sample:Dilute Original density Standard as follow table:
800 pg/ml 5 Standard 150μl Original density Standard+150μl Standard diluent
400 pg/ml 4 Standard 150μl 5 Standard+150μl Standard diluent
200 pg/ml 3 Standard 150μl 4 Standard+150μl Standard diluent
100 pg/ml 2 Standard 150μl 3 Standard +150μl Standard diluent
50pg/ml 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample:Set blank wells separay (blank comparison wells don’t add sample and
HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample
dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is
5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled
water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the
light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color
change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and
within 15min.
Steps description
Standard, Sample diluent
Add Standard, Sample diluent, incubate for 30 min at 37℃.
Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.
Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37℃.
Add Stopp Solution
Read absorbance at 450nm within 15 min
calculate
Calculate
Take the standard density as the horizontal, the OD value for the vertical ,draw the
standard curve on graph paper, Find out the corresponding density according to the sample
OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line
regression equation of the standard curve with the standard density and the OD value ,with the
sample OD value in the equation, calculate the sample density, multiplied by the dilution factor,
the result is the sample actual density.
Important notes
1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in
the room temperature, ELISA plates coated if has not use up after opened, the plate should
be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water helps dissolve
when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the
experimental error. add sample within 5 min, if the number of sample is much , recommend
to use Volley .
4. if the testing material content is excessively higher (The sample OD is bigger than the first
standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution
factor.(×n×5).
5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination must take the
microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to infective material
process.
9. Do not mix reagents with those from other lots.
Storage and validity
1.Storage: 2-8℃.
2.validity: six months人成纖維細(xì)胞生長因子23(FGF-23)Elisa試劑盒說明書

Elisa試劑盒報(bào)價(jià),Elisa試劑盒價(jià)格,Elisa試劑盒說明書,Elisa試劑盒技術(shù),Elisa試劑盒售后,Elisa試劑盒免費(fèi)代測詳情咨詢:
客 服 ,,1278329642,369174963 
電  話:,,
手  機(jī):(陳),(鄧)
傳  真:

[ 打印 ] [ 返回頂部 ] [ 關(guān)閉

| 商鋪首頁 | 公司檔案 | 產(chǎn)品展示 |公司動(dòng)態(tài) | 詢價(jià)留言 | 聯(lián)系我們 | 會(huì)員管理 |
環(huán)保在線 設(shè)計(jì)制作,未經(jīng)允許翻錄必究.Copyright(C) http://www.niunang.cn, All rights reserved.
以上信息由企業(yè)自行提供,信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé),環(huán)保在線對此不承擔(dān)任何保證責(zé)任。
溫馨提示:為規(guī)避購買風(fēng)險(xiǎn),建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。
二維碼

掃一掃訪問手機(jī)站
精品少妇一区二区18-一区二区三区日韩在线播放| av一区免费在线观看-中文字幕日韩国产精品视频| 久久精品人妻一区二区三区极品-久久99热这里只有精品免费| 人妻丝袜中文字幕在线视频-亚洲成av人片一区二区三区| 狠狠狠狠爱精品一二三四区-l舌熟女av国产精品| 性激烈欧美三级在线播放-久久中文字幕人妻少妇| 97人妻精品一区二区三区爱与-日韩精品亚洲专区在线观看| 在线视频成人一区二区-亚洲另类中文字幕在线| 国产av一区二区三区日韩接吻-av网址在线播放网站| 国产免费一区二区三区不-日本少妇免费一区二区三区| 久久99国产综合精品女人-日韩一区二区三区在线不卡| 夜夜久久国产精品亚州av-欧美大屁股一区二区三区| 亚洲一区精品一区在线观看-日本久久久一区二区三区| 亚洲最新国产无人区123-黄片一区二区在线观看| 99精品只有久久精品免费-蜜臀一区二区三区精品久久久| av一区免费在线观看-中文字幕日韩国产精品视频| 国产一级片久久免费看同-麻豆精品尤物一区二区青青| 国产aa视频一区二区三区-国产精品久久久久久久毛片动漫| 久久蜜桃精品一区二区-麻豆视频啊啊啊好舒服| 99久热精品免费观看四虎-亚洲天堂精品视频在线| 色婷婷六月婷婷一区二区-91草草国产欧美在线观看| 狠狠狠狠爱精品一二三四区-l舌熟女av国产精品| 中文字幕日本在线资源-国产+成+人+亚洲欧洲自线| 婷婷亚洲欧美综合丁香亚洲-超刺激国语对白在线视频| 成人免费黄色在线网站-日韩精品一区二区三区四区在线| 人妻互换精品一区二区-夜夜爽一区二区三区视频| 久久久噜噜噜久久狠狠50岁-精品一区二区三区av| 亚洲综合久久综合激情-日韩欧美精品人妻二区少妇| 性都花花世界亚洲综合-日韩av一区二区三区| 白嫩美女娇喘呻吟高潮-久久一区二区三区日产精品| 欧美mv日韩mv视频-熟妇人妻ⅴa精品中文| 五月婷婷免费观看视频-男人操女人下面视频在线免费看| 亚洲另类自拍唯美另类-99国产精品兔免久久| 亚洲av高清一区三区三区-久久人妻夜夜做天天爽| 日本欧美在线视频观看-国产一区二区三区无码下载快播| 成人av一区二区蜜桃-亚洲色图激情人妻欧美| 久久高清超碰av热热久久-国产高清不卡免费视频| 国产精品久久久精品一区-99久久免费精品国产男女性高好| 丝袜美腿人妻连续中出-在线观看日韩三级视频| 国产av剧情护士麻豆-三级国产精品欧美在线观看| 欧美精品一区二区三区爽爽爽-日韩国产精品亚洲经典|