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上海暄泓科學(xué)儀器有限公司
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更新時(shí)間：2024-02-10 17:34:28瀏覽次數(shù)：72次
聯(lián)系我時(shí)，請告知來自 環(huán)保在線C18表面帶電技術(shù)；改善峰形，提高載液量；HPLC到UPLC無縫切換。
XSelect CSH C18 柱是以C18為骨架，相比普通的C18填料，CSH C18具有寬泛的選擇性。CSH技術(shù)能改善峰形，提高載液量。即使裝填在低分散性的柱體內(nèi)，
2010年，沃特世推出了新型表面帶電雜化顆粒(CSH™)技術(shù)，為雜化顆粒表面賦予少量正電荷。利用這一技術(shù)可以使用低離子強(qiáng)度的流動(dòng)相(如0.1%甲酸體系)分析堿性化合物，而仍能獲得的峰形與載量。CSH技術(shù)還允許使用甲酸來替代TFA等離子對試劑，而仍保持帶電分析物的良好峰形，對于質(zhì)譜分析尤為有利。
XSelect CSH C18 柱克服了大多數(shù)現(xiàn)代C18柱在低離子強(qiáng)度流動(dòng)相條件下(如0.1%甲酸體系)因載量所導(dǎo)致的峰形差問題。因帶有嚴(yán)格控制下的、低水平的正表面電荷，該柱為堿性化合物提供行業(yè)的峰形，而無需使用離子對試劑。
按美國藥典方法及中國藥典方法檢測藥物
（Paclitaxel）最初是從紅豆杉科紅豆杉屬（Taxus）植物 的樹皮中提取得到的二萜類化合物，具有抗癌活性，曾 被美國國立癌癥研究所認(rèn)為是近15-20年來腫瘤的最重 要的進(jìn)展。注射液功效主治卵巢癌和乳腺癌及NSCLC 的一線和二線治療。頭頸癌、食管癌，精原細(xì)胞瘤，復(fù)發(fā)非何 金氏淋巴瘤等。因抗癌療效明確，臨床應(yīng)用市場容量 大，原料藥與制劑藥生產(chǎn)需求持續(xù)，對于的新劑型研 究以及對同系物的新藥開發(fā)也一直在持續(xù)進(jìn)行中。 美國藥典方法規(guī)定使用L43全氟苯基柱對[1]以及注射液進(jìn)行含量測定，
系統(tǒng)方法條件如下： 流動(dòng)相：水-乙腈 11:9（即 55:45），如需要時(shí)可適當(dāng)調(diào)整比例。 洗脫：等度，1.5mL/min[1] 色譜柱：5µm, 4.6[1]或4.0[2] mmID x 250mmL，L43（即：PFP，全氟苯基） 檢測：UV 227nm 要求：拖尾因子0.7-1.3范圍內(nèi)[1]；峰的保留時(shí)間在6.0- 10.0min范圍內(nèi)[2] 美國藥典指導(dǎo)性通則USP Chromatography <621>對色譜方法進(jìn)行定義并制定了方法實(shí)施的指導(dǎo)原則，其中也規(guī)定了色譜方 法的調(diào)整范圍，如果檢測方法調(diào)整在此范圍內(nèi)，即視為仍遵 循原方法的法規(guī)要求，只要能夠滿足系統(tǒng)適應(yīng)性要求，就無 需進(jìn)行方法再驗(yàn)證。液相色譜方法的調(diào)整范圍包括不 于如下： - 色譜柱粒徑可減?。ǖ珳p小程度最多為50%） - 柱長度可調(diào)整±70% - 流速可調(diào)整±50% 使用沃特世產(chǎn)品XSelect™ HSS PFP色譜柱(3.5µm, 4.6x150mm，PN186005862)，流速1mL/min，對混標(biāo)進(jìn)行 分離，可達(dá)到系統(tǒng)適應(yīng)性要求，且峰形良好，分離優(yōu)異。柱 規(guī)格與流速的改變在通則<621>的限制范圍之內(nèi)，因此方法仍具有法規(guī)依從性。
品名 | 編號 |
XSelect CSH C18 Column, 130?, 3.5 µm, 2.1 mm X 50 mm, 1/pkg | 186005255 |
XSelect CSH C18 Column, 130?, 3.5 µm, 2.1 mm X 100 mm, 1/pkg | 186005256 |
XSelect CSH C18 Column, 130?, 3.5 µm, 2.1 mm X 150 mm, 1/pkg | 186005257 |
XSelect CSH C18 Column, 130?, 3.5 µm, 1 mm X 150 mm, 1/pkg | 186005251 |
XSelect CSH C18 Column, 130?, 3.5 µm, 2.1 mm X 20 mm, 1/pkg | 186005253 |
XSelect CSH C18 Column, 130?, 3.5 µm, 2.1 mm X 30 mm, 1/pkg | 186005254 |
XSelect CSH C18 Column, 130?, 3.5 µm, 1 mm X 100 mm, 1/pkg | 186005250 |
XSelect CSH C18 Column, 130?, 3.5 µm, 2.1 mm X 75 mm, 1/pkg | 186005644 |
XSelect CSH C18 Column, 130?, 3.5 µm, 4.6 mm X 150 mm, 1/pkg | 186005270 |
XSelect CSH C18 Column, 130?, 3.5 µm, 3 mm X 75 mm, 1/pkg | 186005647 |
XSelect CSH C18 Column, 130?, 3.5 µm, 3 mm X 20 mm, 1/pkg | 186005259 |
XSelect CSH C18 Column, 130?, 3.5 µm, 4.6 mm X 20 mm, 1/pkg | 186005265 |
XSelect CSH C18 Column, 130?, 3.5 µm, 3 mm X 100 mm, 1/pkg | 186005262 |
XSelect CSH C18 Column, 130?, 3.5 µm, 3 mm X 150 mm, 1/pkg | 186005263 |
XSelect CSH C18 Column, 130?, 3.5 µm, 4.6 mm X 75 mm, 1/pkg | 186005268 |
XSelect CSH C18 Column, 130?, 3.5 µm, 4.6 mm X 100 mm, 1/pkg | 186005269 |
XSelect CSH C18 Column, 130?, 3.5 µm, 4.6 mm X 30 mm, 1/pkg | 186005266 |
XSelect CSH C18 Column, 130?, 3.5 µm, 4.6 mm X 50 mm, 1/pkg | 186005267 |
XSelect CSH C18 Column, 130?, 3.5 µm, 3 mm X 30 mm, 1/pkg | 186005260 |
XSelect CSH C18 Column, 130?, 3.5 µm, 3 mm X 50 mm, 1/pkg | 186005261 |
XSelect CSH C18 Column, 130?, 5 µm, 2.1 mm X 30 mm, 1/pkg | 186005273 |
XSelect CSH C18 Column, 130?, 5 µm, 2.1 mm X 50 mm, 1/pkg | 186005274 |
XSelect CSH C18 Column, 130?, 5 µm, 2.1 mm X 100 mm, 1/pkg | 186005275 |
XSelect CSH C18 Column, 130?, 5 µm, 2.1 mm X 150 mm, 1/pkg | 186005276 |
XSelect CSH C18 Column, 130?, 5 µm, 3 mm X 30 mm, 1/pkg | 186005279 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186005290 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 250 mm, 1/pkg | 186005291 |
XSelect CSH C18 Column, 130?, 5 µm, 2.1 mm X 20 mm, 1/pkg | 186005272 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 20 mm, 1/pkg | 186005284 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 30 mm, 1/pkg | 186005286 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 50 mm, 1/pkg | 186005287 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 75 mm, 1/pkg | 186005288 |
XSelect CSH C18 Column, 130?, 5 µm, 4.6 mm X 100 mm, 1/pkg | 186005289 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 4.6 mm X 100 mm, 3/pkg | 186006238 |
XSelect CSH C18 Column, 130?, 5 µm, 3 mm X 50 mm, 1/pkg | 186005280 |
XSelect CSH C18 Column, 130?, 5 µm, 3 mm X 100 mm, 1/pkg | 186005281 |
XSelect CSH C18 Column, 130?, 5 µm, 3 mm X 150 mm, 1/pkg | 186005282 |
XSelect CSH C18 Column, 130?, 5 µm, 3 mm X 250 mm, 1/pkg | 186005283 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 2.1 mm X 50 mm, 3/pkg | 186006233 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 2.1 mm X 150 mm, 3/pkg | 186006785 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 3 mm X 150 mm, 3/pkg | 186006786 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 4.6 mm X 150 mm, 3/pkg | 186006787 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 3 mm X 100 mm, 3/pkg | 186006236 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 4.6 mm X 50 mm, 3/pkg | 186006237 |
XSelect CSH C18 Method Validation Kit, 130?, 2.5 µm, 2.1 mm X 100 mm, 3/pkg | 186006234 |
XSelect CSH C18 Method Validation Kit, 130?, 5 µm, 3 mm X 150 mm, 3/pkg | 186005545 |
XSelect CSH C18 Method Validation Kit, 130?, 5 µm, 4.6 mm X 100 mm, 3/pkg | 186005546 |
XSelect CSH C18 Method Validation Kit, 130?, 5 µm, 4.6 mm X 150 mm, 3/pkg | 186005547 |
XSelect CSH C18 Method Validation Kit, 130?, 5 µm, 4.6 mm X 250 mm, 3/pkg | 186005548 |
XSelect CSH C18 OBD Prep Column, 130?, 5 µm, 19 mm X 100 mm, 1/pkg | 186005421 |
XSelect CSH C18 Method Validation Kit, 130?, 3.5 µm, 3 mm X 150 mm, 3/pkg | 186005540 |
XSelect CSH C18 Method Validation Kit, 130?, 3.5 µm, 4.6 mm X 150 mm, 3/pkg | 186005542 |
XSelect CSH C18 Method Validation Kit, 130?, 3.5 µm, 4.6 mm X 100 mm, 3/pkg | 186005541 |
XSelect CSH C18 Method Validation Kit, 130?, 5 µm, 2.1 mm X 150 mm, 3/pkg | 186005543 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 30 mm X 50 mm, 1/pkg | 186007296 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 19 mm X 150 mm, 1/pkg | 186007293 |
XSelect CSH C18 Method Validation Kit, 130?, 3.5 µm, 2.1 mm X 100 mm, 3/pkg | 186005538 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 19 mm X 250 mm, 1/pkg | 186007294 |
XSelect CSH C18 Method Validation Kit, 130?, 3.5 µm, 3 mm X 100 mm, 3/pkg | 186005539 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 19 mm X 50 mm, 1/pkg | 186007291 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 19 mm X 100 mm, 1/pkg | 186007292 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 30 mm X 150 mm, 1/pkg | 186007299 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 30 mm X 75 mm, 1/pkg | 186007297 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 30 mm X 100 mm, 1/pkg | 186007298 |
XSelect CSH C18 Method Validation Kit, XP Column, 130?, 2.5 µm, 3 mm X 50 mm, 3/pkg | 186006235 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 50 mm X 150 mm, 1/pkg | 186007303 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 50 mm X 250 mm, 1/pkg | 186007304 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 50 mm X 50 mm, 1/pkg | 186007301 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 50 mm X 100 mm, 1/pkg | 186007302 |
XSelect CSH C18 OBD Prep Column, 130?, 10 µm, 30 mm X 250 mm, 1/pkg | 186007300 |
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