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XSelect HSS C18 Column

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提高了HPLC系統(tǒng)的分離效率、方法穩(wěn)定性和分析通量；在低pH條件下的極長(zhǎng)柱壽命

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XSelect HSS C18 柱是超高效通用性C18色譜柱，用于提供對(duì)堿性分析物的優(yōu)異峰形、提高保留(相對(duì)于BEH C18柱)，以及在低pH條件下的極長(zhǎng)柱壽命。XSelect HSS C18 柱所表現(xiàn)出的選擇性與保留性與大多數(shù)現(xiàn)代全封端的硅膠C18色譜柱色譜行為較為相近。

市售低PH流動(dòng)相條件下的鍵合相，不會(huì)像舊的鍵合相一樣有峰型很差的問題，這點(diǎn)依靠的是一種立體的保護(hù)來確保低PH條件下的穩(wěn)定性。

沃特世很清楚顆?；|(zhì)在色譜選擇性和保留特性方面所起的作用：沒有單種顆?；w能夠滿足所有的色譜分析挑戰(zhàn)。 另外，鍵合到不同基體上的同一配體也能產(chǎn)生各種不同的色譜結(jié)果。高強(qiáng)度硅膠 [HSS] 技術(shù)的開發(fā)專門用于與疏水性更 強(qiáng)的 BEH 和 CSH 顆粒的色譜性能相互補(bǔ)。與含亞乙基橋的 BEH 和 CSH 顆粒相比，HSS 顆粒更強(qiáng)的硅醇基活性（99% 硅膠） 可提供明顯的色譜優(yōu)勢(shì)，包括增加極性化合物的保留和顯著的選擇性差異。另外，正如名字所示，HSS 顆粒被設(shè)計(jì)和合 成用于可承受 UPLC 壓力所需的機(jī)械強(qiáng)度。





Development and validation of new RP-HPLC method for the determination of gliclazide in tablet dosage form

A new sensitive and economic analytical method for the analysis of gliclazide in tablet dosage form was developed and validated using commercial HPLC instrument with modern ultra-performance liquid chromatography column. Samples were analysed using Agilent 1100 Series RP-HPLC equipped with a binary pump and auto injector. Samples were injected into Xselect HSS C18 XP column (2.1 × 100 mm, 2.5 μm), 2.5 μm) and the phase consisted of triethylamine buffer 0.3% (pH = 3 adjusted using phosphoric acid) and acetonitrile in the ratio of 50:50 v/v. The flow rate was 0.4 mL/min and the DAD detector was used for the detection and the wavelength was 229 nm. The method was validated according to ICH guidelines. The retention time for gliclazide peak was 3.437 ± 0.41 min with the total run time of 6 min with total solvent consumption of 2.4 mL per run. The method was found linear over the range 0.1-10 μg/mL with coefficient of determination R2 of 0.9997. The recovery was 98.09-100.19 %, and the method showed high precision and repeatability. Degradation pathway of gliclazide was studied following different types of stress degradation which are base, acid, UV, and oxidization. All validated parameters were in the acceptable range of ICH requirements. In conclusion, a new, rapid, sensitive, and economic method was developed and validated for the analysis of gliclazide in tablet dosage form. ? 2017, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.

品名

編號(hào)

XSelect HSS C18 Column, , 100?, 3.5 µm, 1 mm X 150 mm, 1/pkg

186006378

XSelect HSS C18 Column, 100?, 3.5 µm, 3 mm X 30 mm, 1/pkg

186004765

XSelect HSS C18 Column, 100?, 3.5 µm, 3 mm X 50 mm, 1/pkg

186004766

XSelect HSS C18 Column, 100?, 3.5 µm, 4.6 mm X 100 mm, 1/pkg

186004767

XSelect HSS C18 Column, 100?, 3.5 µm, 4.6 mm X 150 mm, 1/pkg

186004768

XSelect HSS C18 Column, 100?, 5 µm, 2.1 mm X 100 mm, 1/pkg

186006392

XSelect HSS C18 Column, 100?, 5 µm, 2.1 mm X 50 mm, 1/pkg

186006391

XSelect HSS C18 Column, 100?, 5 µm, 2.1 mm X 150 mm, 1/pkg

186006393

XSelect HSS C18 Column, 100?, 3.5 µm, 3 mm X 75 mm, 1/pkg

186005642

XSelect HSS C18 Column, , 100?, 5 µm, 2.1 mm X 30 mm, 1/pkg

186006390

XSelect HSS C18 Column, 100?, 3.5 µm, 1 mm X 50 mm, 1/pkg

186006376

XSelect HSS C18 Column, 100?, 3.5 µm, 1 mm X 100 mm, 1/pkg

186006377

XSelect HSS C18 Column, 100?, 5 µm, 3 mm X 150 mm, 1/pkg

186006398

XSelect HSS C18 Column, 100?, 3.5 µm, 3 mm X 150 mm, 1/pkg

186004763

XSelect HSS C18 Column, 100?, 5 µm, 3 mm X 100 mm, 1/pkg

186006397

XSelect HSS C18 Column, 100?, 3.5 µm, 3 mm X 100 mm, 1/pkg

186004762

XSelect HSS C18 Column, 100?, 5 µm, 2.1 mm X 150 mm, 3/pkg

186006411

XSelect HSS C18 Column, 100?, 3.5 µm, 2.1 mm X 100 mm, 1/pkg

186006383

XSelect HSS C18 Column, 100?, 3.5 µm, 2.1 mm X 75 mm, 1/pkg

186006382

XSelect HSS C18 Column, 100?, 3.5 µm, 2.1 mm X 50 mm, 1/pkg

186006381

XSelect HSS C18 Column, 100?, 3.5 µm, 2.1 mm X 30 mm, 1/pkg

186006380

XSelect HSS C18 Column, 100?, 3.5 µm, 4.6 mm X 75 mm, 1/pkg

186006387

XSelect HSS C18 Column, 100?, 3.5 µm, 4.6 mm X 50 mm, 1/pkg

186004772

XSelect HSS C18 Column, 100?, 3.5 µm, 4.6 mm X 30 mm, 1/pkg

186004771

XSelect HSS C18 Column, 100?, 3.5 µm, 2.1 mm X 150 mm, 1/pkg

186006384

XSelect HSS C18 Column, 100?, 3.5 µm, 4.6 mm X 250 mm, 1/pkg

186004770

XSelect HSS C18 Column, 100?, 5 µm, 4.6 mm X 100 mm, 1/pkg

186006403

XSelect HSS C18 Method Validation Kit, 100?, 3.5 µm, 4.6 mm X 100 mm, 3/pkg

186006409

XSelect HSS C18 Method Validation Kit, 100?, 2.5 µm, 2.1 mm X 50 mm, 3/pkg

186006251

XSelect HSS C18 Method Validation Kit, 100?, 2.5 µm, 2.1 mm X 150 mm, 3/pkg

186006794

XSelect HSS C18 Column, 100?, 5 µm, 4.6 mm X 30 mm, 1/pkg

186006401

XSelect HSS C18 Column, 100?, 5 µm, 4.6 mm X 75 mm, 1/pkg

186006402

XSelect HSS C18 Method Validation Kit, 100?, 2.5 µm, 3 mm X 150 mm, 3/pkg

186006795

XSelect HSS C18 Intelligent Speed (IS) Column, 100?, 5 µm, 4.6 mm X 20 mm, 1/pkg

186006400

XSelect HSS C18 Method Validation Kit, 100?, 2.5 µm, 4.6 mm X 150 mm, 3/pkg

186006796

XSelect HSS C18 Column, 100?, 5 µm, 3 mm X 250 mm, 1/pkg

186006399

XSelect HSS C18 Column, 100?, 5 µm, 3 mm X 50 mm, 1/pkg

186006396

XSelect HSS C18 Column, XP Column, 100?, 2.5 µm, 3 mm X 30 mm, 3/pkg

176002622

XSelect HSS C18 Intelligent Speed (IS) Column, 100?, 3.5 µm, 2.1 mm X 20 mm, 1/pkg

186006379

XSelect HSS C18 Column, 100?, 5 µm, 4.6 mm X 50 mm, 1/pkg

186004852

XSelect HSS C18 Column, 100?, 5 µm, 4.6 mm X 250 mm, 1/pkg

186004775

XSelect HSS C18 Method Validation Kit, 100?, 3.5 µm, 4.6 mm X 150 mm, 3/pkg

186006410

XSelect HSS C18 Method Validation Kit, 100?, 5 µm, 4.6 mm, X 250 mm, , 3/pkg

186006416

XSelect HSS C18 Method Validation Kit, 100?, 5 µm, 4.6 mm X 100 mm, 3/pkg

186006414

XSelect HSS C18 Method Validation Kit, 100?, 5 µm, 4.6 mm X 150 mm, 3/pkg

186006415

XSelect HSS C18 Column, 100?, 5 µm, 4.6 mm X 150 mm, 1/pkg

186004773


關(guān)鍵詞：色譜柱
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