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上海暄泓科學(xué)儀器有限公司
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上海暄泓科學(xué)儀器有限公司
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更新時(shí)間：2024-02-11 17:31:06瀏覽次數(shù)：73次
聯(lián)系我時(shí)，請(qǐng)告知來自 環(huán)保在線能耐受二甲基亞砜（DMSO）溶劑，在純化堿性化合物時(shí)，一次進(jìn)樣量增加六十倍。
基于代雜化顆粒技術(shù)的 XTerra® 系列高效液相色譜柱, 可以讓色譜工作者在藥物發(fā)現(xiàn)，方法開發(fā)和純化分離領(lǐng)域使用高pH值流動(dòng)相。
§ XTerra顆粒能耐受二甲基亞砜（DMSO）溶劑，可延長高通量藥物發(fā)現(xiàn)研究中色譜柱的壽命。
§ 寬pH 范圍使得方法開發(fā)研究人員在所需的pH值條件下操作，以獲得性。
§ 高載荷量使得分離純化的科學(xué)家在高pH值條件下，在純化堿性化合物時(shí)，一次進(jìn)樣量增加六十倍。
XTerra® MS、Shield RP和苯基色譜柱將硅膠和聚合物鍵合相的性能與的雜化顆粒技術(shù)相結(jié)合，在顆粒合成中每三個(gè)硅 烷醇中有一個(gè)被替換成甲基基團(tuán)。這個(gè)步驟只能在顆粒合成的 起始階段完成，加入的此甲基基團(tuán)是堿性顆粒骨架的組成部分。 由此可生成機(jī)械強(qiáng)度大的顆粒，適用于高pH值條件下的分離，提 高堿性化合物的上樣量和峰形。
XTerra® RP18和RP8色譜柱將內(nèi)嵌極性基團(tuán)的屏蔽技術(shù)與雜化顆粒技術(shù)結(jié)合起來，可以獲得對(duì)稱的色譜峰形。這一通用型色譜柱以的選擇性為快速方法開發(fā)提供了一個(gè)強(qiáng)有力的工具。同其它所有XTerra® 色譜柱一樣，利用屏蔽技術(shù)的XTerra® RP色譜柱展現(xiàn)了極其優(yōu)秀的水浸潤性，即便是使用99%的水溶液作為流動(dòng)相，亦可獲得穩(wěn)定的色譜結(jié)果。大大拓寬的pH范圍，為選擇性調(diào)節(jié)提供了有效途徑；加上選擇性互補(bǔ)的MS和RP兩種鍵合相更是增加了選擇性的調(diào)節(jié)范圍，是成功進(jìn)行方法開發(fā)的工具。
A simple method is presented for the simultaneous determination of morphine, 6-acetylmorphine, codeine, cocaine, benzoylecgonine, cocaethylene, methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in vitreous humor by high-performance liquid chromatography with photodiode array detector after solid-phase extraction with Oasis® HLB cartridges and dichloromethane as eluent. The chromatographic process was carried out using an XTerra® RP8 column (250 × 4.6 mm i.d., 5 µm particle size) and a phase composed of acetonitrile and pH 6.5 phosphate buffer in gradient mode. A linear response from the detector was obtained within the concentration range of 0.1-4 µg ml(-1) , with correlation coefficients higher than 0.99. The limits of detection were lower than 30 ng ml(-1) for all the drugs studied, the coefficients of variation fluctuated between 0.1 and 12.4%, and the average recoveries were higher than 78% for all the drugs except for EDDP, with a value of 66.4%. Finally, the proposed method was applied to 15 vitreous humor samples coming from individuals who had died from opiate and/or cocaine overdose, showing consumption of cocaine in 14 cases, methadone in five cases and in three cases. Average concentrations of 0.30 µg ml(-1) for morphine, 0.24 µg ml(-1) for 6-acetylmorphine, 0.10 µg ml(-1) for codeine, 0.81 µg ml(-1) for cocaine, 1.26 µg ml(-1) for benzoylecgonine, 0.15 µg ml(-1) for cocaethylene, 0.11 µg ml(-1) for methadone and 0.68 µg ml(-1) for EDDP were obtained.
| 品名 | 編號(hào) |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 19 mm X 150 mm, 1/pkg | 186002258 |
| XTerra RP 8 Column, 125?, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186000493 |
| XTerra RP 8 Column, 125?, 5 µm, 3 mm X 150 mm, 1/pkg | 186000473 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 30 mm X 300 mm, 1/pkg | 186002278 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 50 mm X 250 mm, 1/pkg | 186002850 |
| XTerra RP 8 Column, 125?, 5 µm, 2.1 mm X 150 mm, 1/pkg | 186000457 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 30 mm X 100 mm, 1/pkg | 186001945 |
| XTerra RP 8 Column, 125?, 5 µm, 2.1 mm X 100 mm, 1/pkg | 186000453 |
| XTerra RP 8 Column, 125?, 3.5 µm, 4.6 mm X 50 mm, 1/pkg | 186000435 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 30 mm X 250 mm, 1/pkg | 186002274 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 30 mm X 150 mm, 1/pkg | 186002270 |
| XTerra RP 8 Column, 125?, 5 µm, 4.6 mm X 30 mm, 1/pkg | 186001911 |
| XTerra RP 8 Column, 125?, 5 µm, 4.6 mm X 250 mm, 1/pkg | 186000497 |
| XTerra RP 8 Column, 125?, 5 µm, 3.9 mm X 150 mm, 1/pkg | 186000481 |
| XTerra RP 8 Column, 125?, 5 µm, 2.1 mm X 250 mm, 1/pkg | 186000461 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 19 mm X 100 mm, 1/pkg | 186001937 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 19 mm X 150 mm, 1/pkg | 186002382 |
| XTerra RP 8 Column, 125?, 5 µm, 3 mm X 100 mm, 1/pkg | 186000469 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 50 mm X 150 mm, 1/pkg | 186002846 |
| XTerra RP 8 Column, 125?, 5 µm, 4.6 mm X 50 mm, 1/pkg | 186000485 |
| XTerra RP 8 Column, 125?, 5 µm, 2.1 mm X 50 mm, 1/pkg | 186000449 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 19 mm X 250 mm, 1/pkg | 186002262 |
| XTerra RP 8 Column, 125?, 5 µm, 4.6 mm X 100 mm, 1/pkg | 186000489 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 50 mm X 50 mm, 1/pkg | 186002282 |
| XTerra RP 8 OBD Prep Column, 125?, 10 µm, 19 mm X 300 mm, 1/pkg | 186002266 |
| XTerra RP 8 Prep Guard Cartridge, 125?, 5 µm, 19 mm X 10 mm, 1/pkg | 186001107 |
| XTerra RP 8 Prep Guard Cartridge, 125?, 10 µm, 19 mm X 10 mm, 1/pkg | 186001037 |
| XTerra RP 8 Prep Column, 125?, 5 µm, 7.8 mm X 50 mm, 1/pkg | 186001155 |
| XTerra RP 8 Prep Guard Cartridge, 125?, 10 µm, 7.8 mm X 10 mm, 1/pkg | 186001175 |
| XTerra RP 8 Prep Guard Cartridge, 125?, 5 µm, 7.8 mm X 10 mm, 1/pkg | 186001171 |
| XTerra Shield RP18 Column, 125?, 3.5 µm, 2.1 mm X 150 mm, 1/pkg | 186000410 |
| XTerra Shield RP18 Column, 125?, 3.5 µm, 2.1 mm X 20 mm, 1/pkg | 186001925 |
| XTerra RP18 Prep Guard Cartridge, 125?, 10 µm, 30 mm X 10 mm, 1/pkg | 186006905 |
| XTerra RP18 Prep Guard Cartridge, 125?, 5 µm, 30 mm X 10 mm, 1/pkg | 186006906 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 30 mm X 50 mm, 1/pkg | 186001941 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 30 mm X 75 mm, 1/pkg | 186002390 |
| XTerra RP 8 Prep Column, 125?, 5 µm, 7.8 mm X 100 mm, 1/pkg | 186001159 |
| XTerra RP 8 Prep Column, 125?, 5 µm, 7.8 mm X 150 mm, 1/pkg | 186001478 |
| XTerra RP 8 Prep Guard Cartridge, 125?, 10 µm, 10 mm X 10 mm, 1/pkg | 186001009 |
| XTerra Shield RP18 Cartridge, 125?, 5 µm, 4.6 mm X 150 mm, 1/pkg | 186000580 |
| XTerra RP 8 Prep Guard Cartridge, 125?, 5 µm, 10 mm X 10 mm, 1/pkg | 186001008 |
| XTerra Shield RP18 Column, 125?, 3.5 µm, 2.1 mm X 100 mm, 1/pkg | 186000406 |
| XTerra Shield RP 8 VanGuard Cartridge, 125?, 5 µm, 3.9 mm X 5 mm, 3/pkg | 186007947 |
| XTerra Shield RP 8 VanGuard Cartridge, 125?, 5 µm, 2.1 mm X 5 mm, 3/pkg | 186007945 |
| XTerra Shield RP 8 VanGuard Cartridge, 125?, 3.5 µm, 3.9 mm X 5 mm, 3/pkg | 186007943 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 50 mm X 100 mm, 1/pkg | 186002225 |
| XTerra RP 8 Prep Column, 125?, 10 µm, 7.8 mm X 150 mm, 1/pkg | 186001163 |
| XTerra RP 8 Prep Column, 125?, 10 µm, 7.8 mm X 300 mm, 1/pkg | 186001167 |
| XTerra RP 8 OBD Prep Column, 125?, 5 µm, 50 mm X 50 mm, 1/pkg | 186002221 |
| XTerra Shield RP 8 VanGuard Cartridge, 125?, 3.5 µm, 2.1 mm X 5 mm, 3/pkg | 186007941 |
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