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三鍵鍵合式烷基柱，對(duì)離子化化合物的保留、選擇和靈敏度施加巨大影響

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因其所帶來的的峰對(duì)稱性、柱效和化學(xué)穩(wěn)定性，1.7 μm ACQUITY UPLC BEH C18和C8柱是廣泛適用于各種分析物通用的超高效色譜分離柱。因能在pH 1-12范圍內(nèi)操作，這些三鍵鍵合式烷基柱能夠利用寬pH調(diào)節(jié)對(duì)可離子化化合物的保留、選擇和靈敏度施加巨大影響，同時(shí)還能在低pH和高pH條件下保持優(yōu)異的柱穩(wěn)定性。

This article described the development and full validation of rapid and accurate liquid chromatography method, coupled with tandem mass spectrometry detection, for quantification of dabigatran in human plasma with [ 13C 6]-dabigatran as internal standard. Plasma pretreatment involved a single step protein precipitation with methanol. Separation was performed by ultra performance reversed-phase chromatography on an Acquity UPLC BEH C8 100 mm × 1 mm × 1.7 μm column using a gradient elution mode. The phase was a mix of distilled water containing 0.1% formic acid and methanol containing 0.1% formic acid. Specific multiple reaction monitoring transitions were recorded in positive electrospray ionization. The method was linear over the concentration range of 2–500 μg/L. The intra- and inter-day precision values were below 11.3% and accuracy was within 93.8% and 108.8% for all QC levels (5, 75 and 400 μg/L). The lower limit of quantification was 2 μg/L. Total analysis time was to 10 min including sample preparation.

品名	編號(hào)
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 1/pkg	186003377
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 75 mm, 1/pkg	186005606
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 50 mm, 1/pkg	186002875
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 100 mm, 1/pkg	186004665
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 1/pkg	186002878
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 1/pkg	186002877
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 100 mm, 1/pkg	186002876
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 150 mm, 3/pkg	176001046
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 30 mm, 3/pkg	176001310
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 50 mm, 3/pkg	176000882
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 100 mm, 3/pkg	176000883
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg	176000884
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg	176000885
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg	176001050
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg	176001800
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 30 mm, 1/pkg	186003910
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 50 mm, 1/pkg	186004664
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 30 mm, 1/pkg	186004663
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg	186006020
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg	186004046
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg	186004047
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 150 mm, 3/pkg	176001801
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 75 mm, 1/pkg	186005611
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg	176001799
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 30 mm, 3/pkg	176001798
ACQUITY UPLC BEH C8 VanGuard Pre-column, 130?, 1.7 µm, 2.1 mm X 5 mm, 3/pkg	186003978
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg	186004694
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg	186004693