產品名稱：Halt Phosphatase Inhibitor Cocktail
貨號：Pierce 78420
規(guī)格：1ml
Thermo Scientific Halt Phosphatase Inhibitor Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.
The Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases （PTPs） during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays. Furthermore, the cocktail is compatible with popular protein assays such as the Thermo Scientific Pierce BCA Protein Assay Kit （Part No. 23225） and Bradford-based Pierce Coomassie Plus Assay Kit （Part No. 23236）.
Highlights:
All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
Compatible – works with standard protein assays, including BCA and coomassie （Bradford） assays
Convenient storage – refrigerator （2 to 8°C） storage saves valuable space in the freezer and provides for immediate use
Multiple sizes – select a package size of the 100X cocktail that is ideal for any particular scale and workflow of protein extraction experiment
Product Details:
 Preserved phosphorylation states of three proteins in cell lysate. HeLa cells were lysed in the absence （-） and presence （+） of a 1X concentration of the Thermo Scientific Halt Phosphatase Inhibitor Cocktail. Cell lysates were analyzed by Western blot for total and phosphorylated protein as indicated.