PNGase F from Elizabethkingia miricola

Application

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGase F from Elizabethkingia miricola

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Physical form

Solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

上海力敏實(shí)業(yè)有限公司代理sigma產(chǎn)品，咨詢(xún) 詳細(xì)產(chǎn)品信息