英文名稱：Diaphorase from clostridium kluyveri
其他名稱：黃遞酶；硫辛酰胺脫氫酶；二氫硫辛酰胺脫氫酶；二氫*脫氫酶
CAS號：9001-18-7
分子量：102000～110000
活力：5-20 units/mg protein （biuret）
活力定義： One unit of either "diaphorase" or "lipoyl" dehydrogenase will oxidize 1.0 μmole of β-NADH per min at pH 7.5 at 25 °C, with the corresponding reduction of 2,6-dichlorophenolindophenol
描述：The name "Diaphorase" has been loosely applied to several enzymes which catalyze the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Many different assay procedures and "units" are used. Diaphorases which are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase （β-NADH specific） as well as lipoic and lipoamide dehydrogenase activities. It is reported to be a single protein. However, Massey reports that "diaphorase" is probably a denatured lipoamide dehydrogenase. Pre-incubation of the pig heart preparation with Cu2+ reduces the lipoamide dehydrogenase activity and proportionay increases the β-NADH diaphorase activity. In our laboratory, we have demonstrated this copper effect to some degree on the pig heart enzyme, but no appreciable effect was observed on the Clostridium kluyveri or torula yeast preparations. The lipoamide dehydrogenase:diaphorase ratio is a measure of the denaturation
性狀：淺棕黃色凍干結(jié)晶或呈現(xiàn)熒光的黃綠色粉末，zui適pH值8.5。N-乙基順丁烯二酰亞胺對其有抑制作用
用途：用于科研、實(shí)驗，測定乳酸脫氫酶。除了能催化二氫硫辛酰胺或二氫*脫氫，分別成為硫辛酰胺和*外，也可催化人工電子受體還原，如某些染料、鐵氰酸鹽、醌等。脫去的兩個氫能將FAD（黃素腺嘌呤二核苷酸）還原為FADH2，再傳遞給NAD+（*腺嘌呤二核苷酸+）而形成NADH和H+，該酶主要用于生化試劑
保存：2～8℃避光保存
10549B 心肌黃酶/黃遞酶/硫辛酰胺脫氫酶/二氫硫辛酰胺脫氫酶/二氫*脫氫酶/Diaphorase 9001-18-7 生物技術(shù)級，50u/mg 2KU 1500