當前位置：上海復(fù)祥生物科技有限公司>>ATCC細胞>>CRL-2854 WPMY-1 人正常前列腺基質(zhì)永生化細胞

CRL-2854 WPMY-1 人正常前列腺基質(zhì)永生化細胞

返回列表頁

參考價

面議

具體成交價以合同協(xié)議為準

產(chǎn)品型號CRL-2854 WPMY-1

品牌

廠商性質(zhì)生產(chǎn)商

所在地上海市

在線詢價收藏產(chǎn)品查看聯(lián)系電話

聯(lián)系方式：李先生查看聯(lián)系方式

更新時間：2022-08-03 13:42:16瀏覽次數(shù)：246次

聯(lián)系我時，請告知來自環(huán)保在線

產(chǎn)品分類 品牌分類

  ATCC細胞

全部產(chǎn)品列表

暫無信息

上海復(fù)祥生物科技有限公司

經(jīng)營模式：生產(chǎn)廠家

商鋪產(chǎn)品：197條

所在地區(qū)：上海上海市

聯(lián)系人：李先生（經(jīng)理）

詢價 給他留言

產(chǎn)品簡介

詳細介紹

CRL-2854 WPMY-1 人正常前列腺基質(zhì)永生化細胞 的詳細介紹

CRL-2854 WPMY-1 人正常前列腺基質(zhì)永生化細胞

ATCC^® Number:

CRL-2854™

Price:

Designations:	WPMY-1
Depositors:	MM Webber
Biosafety Level:	2 [Cells containing SV40 viral DNA sequence ]
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	myofibroblast
Source:	Organ: prostate Tissue: stroma Disease: normal
Cellular Products:	fibronectin [89997] smooth muscle alpha-actin vimentin [89997]
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1992
Receptors:	androgen receptor, expressed （ [89997] upregulated upon exposure to androgen）
Tumorigenic:	No
Antigen Expression:	kallikrein 3, KLK3 （prostate specific antigen, PSA）; Homo sapiens [89997]
Cytogenetic Analysis:	At passage 66, a majority of the cells were in the 58-68 range; X, -Y. [89997]
Isoenzymes:	AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 0 PGM1, 2 PGM3, 1
Age:	54 years
Gender:	male
Ethnicity:	Caucasian, White
Comments:	The myofibroblast stromal cell line, WPMY-1, was derived from stromal cells from the same peripheral zone of the histologically normal adult prostate, as that used for RWPE-1 cells （ATCC CRL-11609）. Stromal cells were immortalized with SV40-large-T antigen gene, using a pRSTV plasmid construct. WPMY-1 stromal cells belong to a family of cell lines derived from the same prostate as the epithelial RWPE-1 cells and all of its epithelial derivatives. Because of their derivation from the same peripheral zone of the prostate, the WPMY-1 cell line is especially useful for studies on paracrine and stromal : epithelial interactions. The depositor reports that the RWPE-1 cell line （ATCC CRL-11609）, derived from the same prostate, was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%. Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C Growth Conditions: Subculture cells before or upon reaching confluence. Do not allow cells to become super-confluent.
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline （D-PBS）. Add 2.0 to 3.0 ml of 0.025% Trypsin - 0.26 mM EDTA solution （1:1 dilution of 0.05% Trypsin - 0.53 mM EDTA in D-PBS） to the flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor （or 2% fetal bovine serum in D-PBS） and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum between 4 X 10（3） to 6 X 10（3） viable cells/sq. cm is recommended. Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentraton between 6 X 10（3） and 5 X 10（4） cells/sq. cm. *Subction Ratio:** A subction ratio of 1:3 to 1:6 is recommended Medium Renewal:* Every 48 hours
Preservation:	Freeze medium: Complete growth medium supplemented with an additional 15% fetal bovine serum and 10% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Doubling Time:	38 hours
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020 derived from same individual:ATCC CRL-11609 derived from same individual:ATCC CRL-11610 derived from same individual:ATCC CRL-2849 derived from same individual:ATCC CRL-2850 derived from same individual:ATCC CRL-2852 derived from same individual:ATCC CRL-2851
References:	46793: Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605 46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606 46799: Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420 46950: Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548 53180: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636 53181: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337 53182: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152 53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759 53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497 53193: Achanzar WE, et al. Cadmium induces c-myc, p53, and c-jun expression in normal human prostate epithelial cells as a prelude to apoptosis. Toxicol. Appl. Pharmacol. 164: 291-300, 2000. PubMed: 10799339 53194: Achanzar WE, et al. Cadmium-induced malignant transformation of human prostate epithelial cells. Cancer Res. 61: 455-458, 2001. PubMed: 11212230 53196: Bello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed: 11170142 53197: Webber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724 89996: Quader ST, et al. Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model. Mutat. Res. 496: 153-161, 2001. PubMed: 11551491 89997: Webber MM, et al. A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192, 1999. PubMed: 10383888 89998: Bello-DeOcampo D, et al. The role of alpha 6 beta 1 integrin and EGF in normal and malignant acinar morphogenesis of human prostatic epithelial cells. Mutat. Res. 480-481: 209-217, 2001. PubMed: 11506815 90372: Webber MM, et al. Modulation of the malignant phenotype of human prostate cancer cells by N-（4-hydroxyphenyl）retinamide （4-HPR）. Clin. Exp. Metastasis 17: 255-263, 1999. PubMed: 10432011 90375: Sharp RM, et al. N-（4-hydroxyphenyl）retinamide （4-HPR） decreases neoplastic properties of human prostate cells: an agent for prevention. Mutat. Res. 496: 163-170, 2001. PubMed: 11551492 90376: Carruba G, et al. Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 50: 73-82, 2002. PubMed: 11816015

參考文獻和*培養(yǎng)條件,上有細胞照片,手機xiangfbio.詳情請登陸

http://www.xiangbio.com/歡迎各位老師!
iec-6 大鼠小腸隱窩上皮細胞
馬-達二氏犬腎細胞系 MDCK （NBL-2） CRL-34
鴨子胚胎成纖維細胞　 Duck embryo　 CCL-141
小鼠胚胎成纖維細胞 3T3-L1 CCL-173
人結(jié)腸直腸腺癌 COLO 320DM CCL-220
人結(jié)腸直腸腺癌 NCI-H508 CRL-253
人結(jié)腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細胞 Ramos（RA1） CRL-1596
人淋巴母細胞 T2（174×CEM.T2） CRL-1992
大鼠心肌細胞 H9C2（2-1） CRL-1446
豬鼻甲黏膜成纖維細胞 PT-K75 CRL-2528
小鼠胰腺癌細胞 LTPA CRL-2389
大鼠胰腺癌細胞 DSL-6A/C1 CRL-2132
小鼠骨髓瘤B淋巴細胞 SP2/MIL-6 CRL-2016
大鼠腦間質(zhì)細胞 DI TNC1 CRL-2005
小鼠肌原細胞 C2C12 CRL-1772

CRL-2854 WPMY-1 人正常前列腺基質(zhì)永生化細胞
鵪鶉纖維肉瘤細胞 QT6 CRL-1708
大鼠回腸細胞 IEC-18 CRL-1589
非洲綠猴腎細胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纖維細胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細胞 WPE-int CRL-2888
人正常前列腺成肌纖維細胞 WPMY-1 CRL-2854
人胰腺導(dǎo)管腺癌細胞 PL45 CRL-2558
人胰腺癌細胞 Panc 05.04 CRL-2557
人胰腺癌細胞 Panc 04.03 CRL-2555
人胰腺癌細胞 Panc 02.03 CRL-2553
人胰腺癌細胞 Panc 08.13 CRL-2551
人胰腺癌細胞 Panc 03.27 CRL-2549
人胰腺癌細胞 Panc 10.05 CRL-2547
人肝癌細胞 SNU-398 CRL-2233
人成神經(jīng)瘤細胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細胞 HPAC CRL-2119
人胰腺癌細胞 HPAF-II CRL-1997
人黑色素瘤細胞 A375.S2 CRL-1872
人臍靜脈內(nèi)皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
人膀胱癌細胞 HT-1376 CRL-1472
人正常前列腺上皮細胞 RWPE-2 CRL-11610
人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
小鼠巨噬細胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細胞 A-204 HTB-82
人胰腺癌細胞 Capan-2 HTB-80
人胰腺癌細胞 Capan-1 HTB-79
人乳腺導(dǎo)管癌細胞 MDA-MB-435S HTB-129
人胃癌細胞 Hs 746T HTB-135
人肺腺癌細胞 NCI-H441 [H441] HTB-174

人骨髓瘤細胞 U266 TIB-196
人結(jié)腸癌細胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細胞瘤細胞 BE（2）-C CRL-2268
人腎透明細胞癌細胞 Caki-2 HTB-47
人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細胞系
CRL-5826 NCI-H226 人肺鱗癌細胞系
Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉(zhuǎn)移細胞
U14 小鼠宮頸癌細胞
CRL-9518 L5178Y 小鼠淋巴瘤細胞
HTB-112 HEC-1-A 人子宮內(nèi)膜腺癌細胞
HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細胞
AN3 CA 人子宮內(nèi)膜腺癌細胞
ROS1728 大鼠成骨細胞
CRL-6323 B16F1 小鼠黑色素瘤細胞
HTB-58 SK-MES-1 人肺鱗癌細胞
CCL-127 IMR-32 人神經(jīng)母細胞瘤細胞
HTB-79 Capan-2 人胰腺癌細胞
BALL-1 人B淋巴細胞急性白血病細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344 大鼠肝干細胞
CRL-1715 TM4 正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
CRL-1442 BRL-3A 大鼠肝細胞
BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內(nèi)膜癌細胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細胞

BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞
3AO人卵巢癌細胞
iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質(zhì)母細胞瘤
Renca 小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉(zhuǎn)移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結(jié)腸腺癌細胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內(nèi)腺癌細胞
NCI-H446 人小細胞肺癌細胞
MSTO-211H 人肺癌細胞